高分辨率熔解曲线分析区分中国的河豚鱼种（spp.）。
High-Resolution Melting Analysis of Sequences Distinguishes Pufferfish Species ( spp.) in China.
机构信息
Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
Hangzhou Neoline Technology Co., Ltd., Hangzhou 310004, China.
出版信息
J Agric Food Chem. 2021 Jan 20;69(2):794-804. doi: 10.1021/acs.jafc.0c06584. Epub 2021 Jan 5.
Pufferfish is a traditional, delicious dish in Asia. However, eating wild or improperly processed pufferfish causes serious poisoning. This study aimed to exploit the high-resolution melting (HRM) method for authenticating four species of pufferfish (, , , and ). Candidate DNA barcodes, including the cytochrome c oxidase subunit I (), cytochrome oxidase b (), and the control region (D-loop), were analyzed, with selected as the optimal DNA barcode. An HRM method was developed to identify 57 commercial fish samples in China, including 33 commercial pufferfish products and 24 unlabeled fish products. The findings revealed that the pufferfish products were or , and four samples were detected in unlabeled fish products. These results showed that DNA barcode coupled with HRM analysis was a rapid and efficient tool to identify pufferfish, which might aid in the prevention of consumer fraud or mislabeling of fish products.
河豚是亚洲传统的美味佳肴。然而，食用野生或处理不当的河豚会导致严重中毒。本研究旨在利用高分辨率熔解（HRM）方法鉴定四种河豚（、、、和）。对候选 DNA 条码，包括细胞色素 c 氧化酶亚基 I（）、细胞色素氧化酶 b（）和控制区（D-loop）进行了分析，选择作为最佳 DNA 条码。建立了一种 HRM 方法来鉴定中国的 57 个商业鱼类样本，包括 33 个商业河豚产品和 24 个未标记的鱼类产品。研究结果表明，河豚产品为或，在未标记的鱼类产品中检测到四个样本。这些结果表明，DNA 条码结合 HRM 分析是一种快速有效的鉴定河豚的工具，有助于防止消费者欺诈或鱼类产品的误贴标签。
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