College of Fisheries and Life Science, Dalian Ocean University, Dalian, China.
Key Laboratory of Environment Controlled Aquaculture (KLECA), Ministry of Education, Dalian, China.
Biosci Biotechnol Biochem. 2021 Apr 24;85(5):1088-1096. doi: 10.1093/bbb/zbab002.
In this study, we isolated and characterized HSP70 cDNA from pufferfish (Takifugu rubripes). The 3053 bp full-length TrHSP70 sequence consisted of a 167 bp 5'-UTR (untranslated region), a 2535 bp open reading frame, and a 351 bp 3'-UTR. BLAST analysis revealed that the TrHSP70 shared high similarity with HSP70 sequences in other species. In our study, we set 3 experimental groups as H1 group (20 °C), H2 group (24 °C), and H3 group (28 °C) for checking the expression level of TrHSP70 in T. rubripes. Tissue-specific gene expression results showed that TrHSP70 had higher expression in the intestines than other tissues of the T. rubripes by RT-qPCR. In the experimental group, we found that the expression of TrHSP70 was upregulated in different tissues in the H3 group. The results show that TrHSP70 is a constitutively expressed gene, which plays an important role in maintaining normal physiological function and coping with stress.
在这项研究中，我们从河豚（Takifugu rubripes）中分离并鉴定了 HSP70 cDNA。全长为 3053bp 的 TrHSP70 序列由 167bp 的 5'-UTR（非翻译区）、2535bp 的开放阅读框和 351bp 的 3'-UTR 组成。BLAST 分析表明，TrHSP70 与其他物种的 HSP70 序列具有高度相似性。在我们的研究中，我们设置了 3 个实验组，即 H1 组（20°C）、H2 组（24°C）和 H3 组（28°C），以检查 T. rubripes 中 TrHSP70 的表达水平。组织特异性基因表达结果显示，通过 RT-qPCR 检测，TrHSP70 在河豚的肠道中的表达水平高于其他组织。在实验组中，我们发现 TrHSP70 在 H3 组的不同组织中均呈上调表达。结果表明，TrHSP70 是一个组成型表达基因，在维持正常生理功能和应对应激方面发挥着重要作用。
