衰老引起的精子 DNA 甲基化变化可被围产期阻燃剂的低剂量所修饰。
Aging-induced changes in sperm DNA methylation are modified by low dose of perinatal flame retardants.
机构信息
Department of Environmental Health Sciences, School of Public Health & Health Sciences, University of Massachusetts, 686 North Pleasant Street, Amherst, MA 01003, USA.
Federal Budget Institution of Science Central Research Institute of Epidemiology of The Federal Service on Customers' Rights Protection & Human Well-being Surveillance, Novogireevskaya .3a, Moscow 111123, Russia.
出版信息
Epigenomics. 2021 Feb;13(4):285-297. doi: 10.2217/epi-2020-0404. Epub 2021 Jan 6.
Paternal age is increasing in developed countries. Understanding of aging-related epigenetic changes in sperm is needed as well as factors that modify such changes. Young pubertal and mature rats were exposed perinatally to vehicle or environmental xenobiotic 2,2',4,4'-tetrabromodiphenyl ether. Epididymal sperm was reduced representation bisulfite sequenced. Differentially methylated regions (DMRs) were identified via MethPipe. In control animals, 5319 age-dependent DMRs were identified. Age-related DMRs were enriched for embryonic development. In exposed rats, DNA methylation was higher in young and lower in mature animals then in controls. Sperm methylome undergoes significant age-dependent changes, which may represent a causal link between paternal age and offspring phenotype. Environmental xenobiotics can interfere with the natural process of epigenetic aging.
父龄在发达国家呈上升趋势。需要了解精子与衰老相关的表观遗传变化，以及改变这些变化的因素。幼龄和成熟的大鼠在围产期接受环境污染物 2,2',4,4'-四溴二苯醚的处理。对附睾精子进行重亚硫酸盐测序。通过 MethPipe 鉴定差异甲基化区域 (DMR)。在对照动物中，鉴定出 5319 个与年龄相关的 DMR。年龄相关的 DMR 富含胚胎发育。在暴露的大鼠中，年轻动物的 DNA 甲基化水平较高，而成熟动物的 DNA 甲基化水平较低，均高于对照组。精子甲基组经历了显著的年龄依赖性变化，这可能代表了父龄与后代表型之间的因果关系。环境污染物会干扰表观遗传衰老的自然过程。
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