Smit A B, Vreugdenhil E, Ebberink R H, Geraerts W P, Klootwijk J, Joosse J
Biologisch Laboratorium, Vrije Universiteit, Amsterdam, The Netherlands.
Nature. 1988 Feb 11;331(6156):535-8. doi: 10.1038/331535a0.
Insulin and related peptides are key hormonal integrators of growth and metabolism in vertebrates. There is little biochemical evidence for insulin-related peptides in invertebrates, apart from insects for which definitive structural information on these peptides (prothoracicotropic hormone, PTTH) has recently been obtained. We report here the first complete complementary DNA-derived primary structure of a preproinsulin-related protein from identified neurons in an invertebrate, the mollusc Lymnaea stagnalis. We have demonstrated by in situ hybridization that transcription of the gene for this molluscan insulin-related peptide (MIP) occurs in the cerebral light-green cells, giant neuroendocrine cells involved in the control of growth, as well as in a pair of neuroendocrine cells called the canopy cells. The insulin-related peptide precursor has the same overall structure as its vertebrate counterparts. The discovery of insulin-related peptides in invertebrates substantiates the evidence for a widespread and early evolutionary origin of the insulin superfamily.
胰岛素及相关肽是脊椎动物生长和代谢的关键激素整合因子。除昆虫外，无脊椎动物中几乎没有胰岛素相关肽的生化证据，而昆虫的这些肽（促前胸腺激素，PTTH）最近已获得确切的结构信息。我们在此报告了来自无脊椎动物——软体动物椎实螺已鉴定神经元的胰岛素原相关蛋白的首个完整互补DNA推导的一级结构。我们通过原位杂交证明，这种软体动物胰岛素相关肽（MIP）基因的转录发生在脑浅绿色细胞、参与生长控制的巨大神经内分泌细胞以及一对称为冠细胞的神经内分泌细胞中。胰岛素相关肽前体与其脊椎动物对应物具有相同的整体结构。无脊椎动物中胰岛素相关肽的发现证实了胰岛素超家族广泛且早期进化起源的证据。
