KU Leuven, Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, Herestraat 49, O&N2, PB, 923, 3000, Leuven, Belgium; Mekelle University, College of Health Sciences, School of Pharmacy, P.O. Box 1871, Mekelle, Ethiopia.
Mekelle University, College of Health Sciences, School of Pharmacy, P.O. Box 1871, Mekelle, Ethiopia.
J Pharm Biomed Anal. 2021 Feb 20;195:113834. doi: 10.1016/j.jpba.2020.113834. Epub 2020 Dec 9.
Aloe leaf latex is a commonly used plant preparation in traditional medicine. However, quality control on the content of medicinally important constituents is often limited. Hence, establishing a reliable quality control method to identify and quantify bioactive markers is important to ensure safety and efficacy. In the present study, a novel liquid chromatographic (LC) method was developed and validated for efficient analysis of bioactive markers to evaluate the quality of aloe leaf latex. Quantification of marker compounds was possible in only 7 min on a monolithic column using gradient elution with 0.1 % formic acid in acetonitrile and water as mobile phases. The major compounds (aloins A and B) could be baseline separated together with related compounds within 10 min. The method showed excellent linearity with determination coefficients (r) of 0.9999. Detection limits were 0.017 and 0.013 μg/mL, while quantification limits were 0.057 and 0.043 μg/mL for aloin A and aloin B, respectively. Relative standard deviation (RSD) values for intra- and inter-day precision were less than 2% and recoveries for both aloins were close to 100 %. The robustness was evaluated using an experimental design. The method was applied to some aloe leaf latex samples from Ethiopia. Aloin contents varied from 14 to 35 % and two unknown peaks were tentatively identified as aloinoside and microdontin.
芦荟叶乳胶是传统医学中常用的植物制剂。然而，对药用成分含量的质量控制通常是有限的。因此，建立一种可靠的质量控制方法来识别和定量生物活性标志物对于确保安全性和有效性非常重要。在本研究中，开发并验证了一种新的液相色谱（LC）方法，用于有效分析生物活性标志物，以评估芦荟叶乳胶的质量。在使用乙腈和水作为流动相的梯度洗脱中，仅在 7 分钟内即可在整体柱上定量分析标记化合物。主要化合物（芦荟 A 和 B）可以与 10 分钟内的相关化合物一起基线分离。该方法表现出优异的线性，决定系数（r）为 0.9999。检测限分别为 0.017 和 0.013 μg/mL，而芦荟 A 和 B 的定量限分别为 0.057 和 0.043 μg/mL。日内和日间精密度的相对标准偏差（RSD）值均小于 2%，两种芦荟的回收率均接近 100%。通过实验设计评估了稳健性。该方法应用于来自埃塞俄比亚的一些芦荟叶乳胶样品。芦荟含量从 14%到 35%不等，两个未知峰被推测为芦荟苷和微蒽酮。
