Characterization and Evaluation of Antioxidant Activity of Reynolds.

BACKGROUND

The role of free radical reactions in disease pathology is well known to be involved in many acute and chronic disorders in human beings, such as diabetes, atherosclerosis, aging, immunosuppression and neurodegeneration. The search for new drugs of plant origin becomes increasingly urgent due to drug resistance. is an endemic species traditionally used for the treatment of infectious and chronic diseases.

AIM

This study was conducted to evaluate free radical scavenging activities of leaf latex of its isolated compounds.

METHODS

The leaf latex of was subjected to preparative thin-layer chromatography to afford three compounds. Free radical scavenging activities of the leaf latex and its constituents was carried out using a 2, 2-diphenyl-1-picrylhydrazyl method.

RESULTS

Phytochemical investigation of the leaf latex by prepartive thin layer chromatography led to the isolation of three compounds, identified as microdontin A/B (), aloin A/B () and aloinoside A/B (). The results showed that the leaf latex had a strong free radical scavenging activity reaching a maximum of 84.3% at a concentration of 100 μg/mL, and with an IC value of 25.3 ± 2.45 μg/mL ( < 0.05). Among the isolated compounds, microdontin A/B () was found to have the strongest free radical scavenging activity with an IC value of 0.07 ± 0.005 mμ, followed by aloinoside A/B (IC = 0.13 ± 0.01 mM) and aloin A/B (IC = 0.15 ± 0.02 mM).

CONCLUSION

The traditional medicinal practice of the leaf latex may be due to the antioxidant activities of the leaf latex of and the isolated compounds.