Fujimoto M, Weaker F J, Herbert D C, Sharp Z D, Sheridan P J, Story J L
Department of Surgery, University of Texas Health Science Center at San Antonio 78284.
Neurology. 1988 Feb;38(2):289-93. doi: 10.1212/wnl.38.2.289.
We determined which viral oncogenes (v-sis, v-myc, and v-fos) were expressed in five primary human brain tumors of neuroectodermal origin (two glioblastomas multiforme, one medulloblastoma, one cystic cerebellar astrocytoma, and one ganglioglioma) and which of these oncogenes is correlated with malignancy. Using the dot hybridization technique, we determined the relative amounts of mRNA coded by these genes using the same nitrocellulose filter. The v-myc probe showed a 4- to 12-fold greater hybridization to the mRNA from two glioblastomas and the medulloblastoma (malignant group) than the mRNA from the cystic cerebellar astrocytoma or the ganglioglioma (benign group). In contrast, RNA hybridizing to v-sis and v-fos were accumulated to a greater extent in the benign tumors. These data suggest that the amount of myc expression may be correlated with the degree of malignancy of brain tumors of neuroectodermal origin.
我们确定了哪些病毒癌基因（v-sis、v-myc和v-fos）在5例神经外胚层起源的原发性人脑肿瘤（2例多形性胶质母细胞瘤、1例髓母细胞瘤、1例囊性小脑星形细胞瘤和1例节细胞胶质瘤）中表达，以及这些癌基因中的哪些与恶性程度相关。使用点杂交技术，我们在同一张硝酸纤维素滤膜上测定了这些基因编码的mRNA的相对含量。v-myc探针与来自2例胶质母细胞瘤和髓母细胞瘤（恶性组）的mRNA的杂交信号比来自囊性小脑星形细胞瘤或节细胞胶质瘤（良性组）的mRNA强4至12倍。相反，与v-sis和v-fos杂交的RNA在良性肿瘤中积累程度更高。这些数据表明，myc的表达量可能与神经外胚层起源的脑肿瘤的恶性程度相关。
