MacGregor D N, Ziff E B
Department of Pediatrics, New York University Medical Center, New York 10016.
Pediatr Res. 1990 Jul;28(1):63-8. doi: 10.1203/00006450-199007000-00014.
Medulloblastoma is a rare brain tumor usually occurring in late childhood or early adolescence. Little is known regarding the cell of origin or cellular events leading to its malignant transformation. We have studied the expression of developmentally regulated mRNA in tumor samples by the Northern hybridization assay to determine a relative stage at which a block to further differentiation occurs. In a series of five medulloblastoma tumors analyzed, only one of eight markers for cellular differentiation, the glial fibrillary acidic protein mRNA, was expressed at significant levels. Interestingly, three of six medulloblastoma tumor samples were found to have elevated levels of c-myc mRNA. In one of these tumors, we have found evidence of mutation of the c-myc protooncogene. We discuss possible mechanisms of c-myc activation in medulloblastoma tumors.
髓母细胞瘤是一种罕见的脑肿瘤，通常发生在儿童晚期或青少年早期。关于其起源细胞或导致其恶性转化的细胞事件，我们所知甚少。我们通过Northern杂交试验研究了肿瘤样本中发育调控mRNA的表达，以确定进一步分化受阻的相对阶段。在分析的一系列五个髓母细胞瘤肿瘤中，细胞分化的八个标志物中只有一个，即胶质纤维酸性蛋白mRNA，有显著表达。有趣的是，六个髓母细胞瘤肿瘤样本中有三个被发现c-myc mRNA水平升高。在其中一个肿瘤中，我们发现了c-myc原癌基因突变的证据。我们讨论了髓母细胞瘤肿瘤中c-myc激活的可能机制。
