Sobhani-Eraghi A, Panahi M, Shirani A, Pazoki-Toroudi H
Department of Orthopaedic Surgery, Iran University of Medical Sciences, Tehran, Iran.
Department of Pathology, Iran University of Medical Sciences, Tehran, Iran.
Malays Orthop J. 2020 Nov;14(3):155-160. doi: 10.5704/MOJ.2011.024.
Doxycycline is a commonly used antibiotic that is also a potent inhibitor of matrix metalloproteinase (MMPs). The use of doxycycline in repairing tendon lesions has been previously investigated and conflicting findings have been reported on its effectiveness. In this study, we sought to evaluate the effects of exposure to doxycycline on Achilles tendon repair.
Twenty healthy rats of the same breed and gender were randomly assigned to two groups of sham, and Doxycycline group therapy. The rats underwent a surgical intervention in which a 2mm incision was performed on the lateral sides of the right Achilles tendons. The treatment group received oral gavage administrations of 50mg/kg/day of doxycycline for 30 days. After this duration, tissue samples were taken from the site of the injuries, which were then histologically evaluated for alignment of the collagen fibres, inflammation reaction, cellular density, and fibroblastic activity.
The histological assessment of the tissue samples, revealed significant changes in the repaired tissues of the treatment group in comparison to the sham group; namely more irregularity in the alignment of the collagen fibres, increased cellular density, and increased fibroblastic activity. However, only the alignment of the collagen fibres reached the statistical significance.
The results of this study indicate that exposure to doxycycline may result in the improvement of repair of the Achilles tendon injuries, especially collagen filament integrity.
强力霉素是一种常用抗生素，也是基质金属蛋白酶（MMPs）的有效抑制剂。此前已对强力霉素在修复肌腱损伤中的应用进行了研究，但其有效性的研究结果相互矛盾。在本研究中，我们试图评估强力霉素对跟腱修复的影响。
将20只相同品种和性别的健康大鼠随机分为假手术组和强力霉素治疗组。对大鼠进行手术干预，在右跟腱外侧做一个2mm的切口。治疗组连续30天每天经口灌胃给予50mg/kg的强力霉素。在此期间结束后，从损伤部位采集组织样本，然后对其进行组织学评估，观察胶原纤维排列、炎症反应、细胞密度和成纤维细胞活性。
组织样本的组织学评估显示，与假手术组相比，治疗组修复组织有显著变化；即胶原纤维排列更不规则、细胞密度增加和成纤维细胞活性增加。然而，只有胶原纤维排列达到统计学意义。
本研究结果表明，使用强力霉素可能会改善跟腱损伤的修复，尤其是胶原纤维的完整性。
