Zhang Jinchi, Zhang Xiaowei, Li Wei, Guo Jing, Yang Liqun, Yan Guangqi
Department of Biomaterials, Shengjing Hospital of China Medical University, Shenyang, China.
NHC Key Laboratory of Reproductive Health and Medical Genetics (China Medical University), Liaoning Research Institute of Family Planning (The Affiliated Reproductive Hospital of China Medical University), Shenyang, China.
Front Bioeng Biotechnol. 2023 Feb 24;11:1135248. doi: 10.3389/fbioe.2023.1135248. eCollection 2023.
In this study, Poly (trimethylene carbonate)/Doxycycline hydrochloride (PTMC/DH) films were introduced to repair the Achilles tendon defects for the first time. (PTMC/DH) films with different DH content of 10, 20, and 30% (w/w) were prepared by solvent casting. The and drug release of the prepared PTMC/DH films was investigated. The results of drug release experiments showed that the PTMC/DH films released effective concentrations of doxycycline for more than 7 and 28 days and , respectively. The results of antibacterial activity experiments showed diameters of 25.00 ± 1.00 mm, 29.33 ± 1.15 mm, and 34.67 ± 1.53 mm, respectively, for the inhibition zones produced by the release solutions of PTMC/DH films with 10, 20 and 30% (w/w) DH at 2 h, indicating that the drug-loaded films could inhibit Staphylococcus aureus well. After treatment, the Achilles tendon defects have recovered well, as indicated by the more robust biomechanical properties and the lower fibroblast density of the repaired Achilles tendons. Pathology revealed that the pro-inflammatory cytokine, IL-1β, and the anti-inflammatory factor, TGF-β1, peaked in the first three days and gradually decreased as the drug was released more slowly. These results demonstrated that the PTMC/DH films have great potential for regenerating Achilles tendon defects.
在本研究中，首次引入聚碳酸三亚甲基酯/盐酸多西环素（PTMC/DH）薄膜来修复跟腱缺损。通过溶剂浇铸法制备了DH含量分别为10%、20%和30%（w/w）的（PTMC/DH）薄膜。对制备的PTMC/DH薄膜的 和药物释放情况进行了研究。药物释放实验结果表明，PTMC/DH薄膜分别在7天和28天以上释放出有效浓度的多西环素。抗菌活性实验结果表明，含10%、20%和30%（w/w）DH的PTMC/DH薄膜释放液在2小时产生的抑菌圈直径分别为25.00±1.00毫米、29.33±1.15毫米和34.67±1.53毫米，表明载药薄膜能很好地抑制金黄色葡萄球菌。治疗后，跟腱缺损恢复良好，修复后的跟腱生物力学性能更强，成纤维细胞密度更低。病理学显示，促炎细胞因子IL-1β和抗炎因子TGF-β1在前三天达到峰值，并随着药物释放变慢而逐渐下降。这些结果表明，PTMC/DH薄膜在再生跟腱缺损方面具有巨大潜力。
