Agnes Alison, Long Audi, Best Samantha, Lobner Doug
Department of Biomedical Sciences, Marquette University, Milwaukee, USA.
Department of Orthodontics, Marquette University, Milwaukee, USA.
Eur Endod J. 2017 May 23;2(1):1-6. doi: 10.5152/eej.2017.17001. eCollection 2017.
Direct pulp capping involves covering exposed pulp to preserve its viability. Calcium hydroxide materials have traditionally been the most commonly used pulp capping compounds; however, they can be toxic, and their success rate in pulp capping is variable. Recently, the compound mineral trioxide aggregate (MTA) has gained wide use for pulp capping. One advantage of MTA is its low toxicity. However, the effects of MTA and calcium hydroxide compounds on the toxicities of other dental materials have not been tested. The aim of this study is to determine whether different pulp capping materials can alter the toxicity of composite restoration materials.
We used cultured human dental pulp cells to test the toxicities of the calcium hydroxide pulp capping material Dycal and MTA. We then tested the abilities of these compounds to alter the toxicity of the composite materials Durafill and Flow Line and to induce oxidative stress.
As expected, Dycal demonstrated toxicity, while MTA did not. However, when cells were exposed to subtoxic amounts of Dycal or MTA, then exposed to Durafill or Flow Line, changes in the composite materials induced toxicity. Treatment with Dycal had no effect on the toxicity of Durafill, but significantly attenuated the toxicity of Flow Line; meanwhile, MTA significantly enhanced the toxicity of Durafill but had no effect on the toxicity of Flow Line. Early changes in oxidative stress were correlated with later changes in cell death. Statistical calculations were performed using one-way ANOVA followed by the Bonferroni t-test. P-values <0.05 were considered to indicate significant differences.
The results suggest that when choosing a pulp capping material, one factor that should be considered is the impact of that compound on the toxicity of the composite material used for restoration.
直接盖髓术是指覆盖暴露的牙髓以维持其活力。氢氧化钙材料一直是最常用的盖髓剂；然而，它们可能具有毒性，并且其盖髓成功率存在差异。近年来，复合三氧化矿物凝聚体（MTA）在盖髓术中得到了广泛应用。MTA的一个优点是其低毒性。然而，MTA和氢氧化钙化合物对其他牙科材料毒性的影响尚未得到测试。本研究的目的是确定不同的盖髓材料是否会改变复合树脂修复材料的毒性。
我们使用培养的人牙髓细胞来测试氢氧化钙盖髓材料Dycal和MTA的毒性。然后我们测试了这些化合物改变复合树脂材料Durafill和Flow Line的毒性以及诱导氧化应激的能力。
正如预期的那样，Dycal表现出毒性，而MTA没有。然而，当细胞暴露于亚毒性剂量的Dycal或MTA，然后再暴露于Durafill或Flow Line时，复合树脂材料的变化会诱导毒性。用Dycal处理对Durafill的毒性没有影响，但显著降低了Flow Line的毒性；同时，MTA显著增强了Durafill的毒性，但对Flow Line的毒性没有影响。氧化应激的早期变化与细胞死亡的后期变化相关。使用单因素方差分析，随后进行Bonferroni t检验进行统计计算。P值<0.05被认为表明存在显著差异。
结果表明，在选择盖髓材料时，应考虑该化合物对用于修复的复合树脂材料毒性的影响。
