van der Waal Suzette V, de Soet Johannes J, Wesselink Paul R, Crielaard Wim
Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands.
Department of Cariology, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands.
Eur Endod J. 2017 Nov 29;2(1):1-5. doi: 10.14744/eej.2017.17022. eCollection 2017.
To investigate the influence of calcium hydroxide (Ca(OH)) on susceptibility to disinfection with sodium hypochlorite (NaOCl) of biofilm bacteria.
Monospecies biofilms of eight strains were subjected to a 2-h challenge with Ca(OH). After a recovery phase, the biofilms were treated with a concentration of NaOCl that was lower than the minimum inhibitory concentration. In a metabolic assay, the efficacy of NaOCl disinfection in Ca(OH)-challenged biofilms and unchallenged biofilms was evaluated. The data were analyzed with Mann-Whitney U and Kruskall- Wallis tests. A P value of less than 0.05 was considered statistically significant.
There were marginal differences in susceptibility to NaOCl among the strains. After the Ca(OH) challenge, seven strains remained equally susceptible to NaOCl disinfection whereas one strain became more resistant to NaOCl (P=0.03).
After a Ca(OH) challenge, in general remained equally susceptible to disinfection with NaOCl.
研究氢氧化钙（Ca(OH)₂）对生物膜细菌对次氯酸钠（NaOCl）消毒敏感性的影响。
对8株单菌种生物膜进行2小时的氢氧化钙处理。在恢复阶段后，用低于最低抑菌浓度的次氯酸钠浓度处理生物膜。在代谢试验中，评估次氯酸钠在经氢氧化钙处理的生物膜和未经处理的生物膜中的消毒效果。数据采用曼-惠特尼U检验和克鲁斯卡尔-沃利斯检验进行分析。P值小于0.05被认为具有统计学意义。
各菌株对次氯酸钠的敏感性存在微小差异。经氢氧化钙处理后，7株菌株对次氯酸钠消毒的敏感性保持不变，而1株菌株对次氯酸钠的耐药性增强（P = 0.03）。
经氢氧化钙处理后，总体上对次氯酸钠消毒的敏感性保持不变。
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