Carbon Composite Materials Research Center, Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), 92 Chudong-ro, Bongdong-eup, Wanju-gun, Jeollabuk-do, 55324, Republic of Korea.
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 2921, Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Anal Bioanal Chem. 2021 Feb;413(4):1193-1202. doi: 10.1007/s00216-020-03083-9. Epub 2021 Jan 6.
Carbon fiber (CNF), prepared by carbonization of electrospun polyacrylonitrile (PAN) fibers, is systematically investigated as a mediator to replace conventional organic matrices for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS). CNF exhibits a high salt tolerance, sensitivity, and resolution for organic matrix-free laser desorption/ionization time-of-flight mass spectrometry (LDI-MS) analysis of various analytes under both positive and negative ionization modes. Especially, saccharides, a neutral molecule having low negative ionization efficiency, are successfully detected with CNF. Taken together, this study clearly demonstrates CNF is a promising material to develop an efficient and universal platform for LDI-MS analysis regardless of preferential ionization modes of analytes. Graphical abstract.
碳纤维（CNF）是通过电纺聚丙烯腈（PAN）纤维碳化制备的，被系统地研究作为一种介体来替代传统的有机基质，用于基质辅助激光解吸/电离飞行时间质谱（MALDI-MS）。CNF 表现出对有机基质辅助激光解吸/电离飞行时间质谱（LDI-MS）分析的高盐容忍度、灵敏度和分辨率，对于正离子和负离子模式下的各种分析物都适用。特别是，对于具有低负离子效率的中性分子糖，也可以用 CNF 成功检测到。总的来说，这项研究清楚地表明，CNF 是一种很有前途的材料，可以开发出高效且通用的 LDI-MS 分析平台，而不受分析物优先的电离模式的影响。
