Universidade Federal do Pampa (UNIPAMPA), Campus Itaqui, Programa de Pós-Graduação em Bioquímica (PPGBioq), Rua Joaquim de Sá Britto, s/n - Bairro Promorar, Itaqui, RS, CEP 97650-000, Brazil.
Universidade Federal do Pampa, Campus Uruguaiana, Programa de Pós-Graduação em Bioquímica (PPGBioq), BR-472 Km 7, Uruguaiana, RS, CEP 97500-970, Brazil.
Environ Sci Pollut Res Int. 2021 Apr;28(14):17519-17531. doi: 10.1007/s11356-020-11848-z. Epub 2021 Jan 5.
Manganese (Mn) is an essential element that, in excess, seems to be involved in the development of different neurodegenerative conditions. Gamma-oryzanol (Ory) was previously reported to possess antioxidant and neuroprotective properties. Thus, we conducted this study to test the hypothesis that Ory can also protect flies in an Mn intoxication model. Adult wild-type flies were fed over 10 days with Mn (5 mM) and/or Ory (25 μM). Flies treated with Mn had a decrease in locomotor activity and a higher mortality rate compared to those in controls. Mn-treated flies also had a significant increase in acetylcholinesterase (AChE) activity, in Mn accumulation and in oxidative stress markers. Moreover, flies treated with Mn exhibited a significant decrease in dopamine levels and in tyrosine hydroxylase activity, as well as in mitochondrial and cellular viability. Particularly important, Ory protected against mortality and avoided locomotor and biochemical changes associated with Mn exposure. However, Ory did not prevent the accumulation of Mn. The present results support the notion that Ory effectively attenuates detrimental changes associated with Mn exposure in Drosophila melanogaster, reinforcing its neuroprotective action/potential.
锰（Mn）是一种必需元素，过量的锰似乎与多种神经退行性疾病的发展有关。γ-谷维素（Ory）先前被报道具有抗氧化和神经保护作用。因此，我们进行了这项研究，以验证 Ory 也可以在 Mn 中毒模型中保护果蝇这一假设。成年野生型果蝇在 10 天以上的时间内用 Mn（5 mM）和/或 Ory（25 μM）喂养。与对照组相比，用 Mn 处理的果蝇的运动活性降低，死亡率更高。与对照组相比，Mn 处理的果蝇乙酰胆碱酯酶（AChE）活性、Mn 积累和氧化应激标志物显著增加。此外，用 Mn 处理的果蝇多巴胺水平和酪氨酸羟化酶活性以及线粒体和细胞活力显著降低。特别重要的是，Ory 可防止死亡率的发生，并避免与 Mn 暴露相关的运动和生化变化。然而，Ory 并不能阻止 Mn 的积累。本研究结果支持这样一种观点，即 Ory 可有效减轻果蝇中与 Mn 暴露相关的有害变化，从而增强其神经保护作用/潜力。
