Department of Biochemistry, Federal University of Technology, P.M.B. 704 Akure, Nigeria.
Department of Biomedical Technology, Federal University of Technology, P.M.B. 704 Akure, Nigeria.
Oxid Med Cell Longev. 2018 Dec 30;2018:8952646. doi: 10.1155/2018/8952646. eCollection 2018.
Metal-induced toxicity in fruit fly ( is one of the established models for studying neurotoxicity and neurodegenerative diseases. Phytochemicals, especially alkaloids, have been reported to exhibit neuroprotection. Here, we assessed the protective effect of alkaloid extract from African Jointfir ( leaf on manganese- (Mn-) induced toxicity in wild type fruit fly. Flies were exposed to 10 mM Mn, the alkaloid extract and cotreatment of Mn plus extract, respectively. The survival rate and locomotor performance of the flies were assessed 5 days posttreatment, at which point the flies were homogenized and assayed for acetylcholinesterase (AChE) activity, nitric oxide (NO), and reactive oxygen species (ROS) levels. Results showed that the extract significantly reverted Mn-induced reduction in the survival rate and locomotor performance of the flies. Furthermore, the extract counteracted the Mn-induced elevation in AChE activity, NO, and ROS levels. The alkaloid extract of the African Jointfir leaf may hence be a source of useful phytochemicals for the development of novel therapies for the management of neurodegeneration.
金属诱导的果蝇毒性（是研究神经毒性和神经退行性疾病的成熟模型之一。植物化学物质，特别是生物碱，已被报道具有神经保护作用。在这里，我们评估了来自非洲 Jointfir（叶子的生物碱提取物对锰（Mn）诱导的野生型果蝇毒性的保护作用。分别将果蝇暴露于 10mM Mn、生物碱提取物和 Mn 加提取物的共处理中。在处理后 5 天评估了果蝇的存活率和运动性能，此时将果蝇匀浆并测定乙酰胆碱酯酶（AChE）活性、一氧化氮（NO）和活性氧（ROS）水平。结果表明，该提取物显著逆转了 Mn 诱导的果蝇存活率和运动性能下降。此外，该提取物对抗了 Mn 诱导的 AChE 活性、NO 和 ROS 水平升高。因此，非洲 Jointfir 叶子的生物碱提取物可能是开发新型神经退行性疾病治疗方法的有用植物化学物质的来源。
