Ankaiah D, Mitra S, Srivastava D, Sivagnanavelmurugan M, Ayyanna R, Jha N, Venkatesan A
Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry, India.
J Appl Microbiol. 2021 Aug;131(2):949-963. doi: 10.1111/jam.14991. Epub 2021 Jan 25.
The present study aims to evaluate the potential antioxidant and antiproliferative properties of probiotic bacterial isolates Weissella cibaria p3B, Bacillus subtilis CS, and Bacillus tequilensis CL, isolated from South Indian fermented tomato pickle (homemade) and gut content of indigenous country chicken.
The bacterial isolates exhibited antimicrobial activity against food-borne, human pathogenic bacteria, along with better survival under different bile and acidic conditions, hydrophobicity towards several hydrocarbons, and adherence to intestinal epithelial cells (INT-407 cells). Also, the intact cell (IC) mixture of the three species showed better DPPH, ABTS, and Fe chelating activity as compared to the individual IC or cell extract (CE) activity. Among the three bacterial species, W. cibaria p3B revealed maximum antiproliferative activity against HeLa and Caco-2 cancer cells, all of which were nontoxic to INT-407 cells. Apart from being non-hemolytic, the bacterial isolates did not display any necrotic inhibition in HeLa and Caco-2 cells. The cell free supernatant (CFS) of the three bacterial isolates were tested for the production of antimicrobial peptides or bacteriocins. It found that the CFS of bacterial isolates was stable at various temperature, pH and sensitive to proteolytic enzymes confirms protenoius in nature of the antimicrobil peptides or bacteriocins.
The bacterial isolates showed promising antimicrobial, antioxidant as well as antiproliferative activities with better survival ability at different pH and bile concentrations. The three bacterial isolates were able to produce potential antimicrobial peptides or bacteriocins.
These results indicate better compatibility of our bacterial isolates against synthetic drugs to avoid adverse side effects and can be processed as dietary supplements against food and human pathogens. They can also provide antioxidative and antiproliferative benefits to humans and animals.
本研究旨在评估从印度南部发酵番茄泡菜（自制）和本地土鸡肠道内容物中分离出的益生菌菌株西巴魏斯氏菌p3B、枯草芽孢杆菌CS和特基拉芽孢杆菌CL的潜在抗氧化和抗增殖特性。
这些细菌分离株对食源性病原体和人类病原菌具有抗菌活性，在不同胆汁和酸性条件下具有更好的生存能力，对几种碳氢化合物具有疏水性，并能黏附于肠上皮细胞（INT-407细胞）。此外，与单个完整细胞（IC）或细胞提取物（CE）活性相比，这三种菌株的完整细胞混合物表现出更好的DPPH、ABTS和铁螯合活性。在这三种细菌中，西巴魏斯氏菌p3B对HeLa和Caco-2癌细胞显示出最大的抗增殖活性，且对INT-407细胞均无毒。除了不溶血外，这些细菌分离株在HeLa和Caco-2细胞中未表现出任何坏死抑制作用。对这三种细菌分离株的无细胞上清液（CFS）进行了抗菌肽或细菌素产生的测试。结果发现，细菌分离株的CFS在不同温度、pH下稳定，且对蛋白水解酶敏感，证实了抗菌肽或细菌素的蛋白质性质。
这些细菌分离株表现出有前景的抗菌、抗氧化以及抗增殖活性，在不同pH和胆汁浓度下具有更好的生存能力。这三种细菌分离株能够产生潜在的抗菌肽或细菌素。
这些结果表明我们的细菌分离株与合成药物具有更好的兼容性，可避免不良副作用，并可作为针对食品和人类病原体的膳食补充剂进行加工。它们还可为人类和动物提供抗氧化和抗增殖益处。
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