Department of Nano-Bio Convergence, Korea Institute of Materials Science (KIMS), Changwon, Gyeongnam 51508, Republic of Korea.
Advanced Materials Engineering, University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
ACS Appl Mater Interfaces. 2021 Jan 20;13(2):3024-3032. doi: 10.1021/acsami.0c18892. Epub 2021 Jan 6.
A wearable surface-enhanced Raman scattering (SERS) sensor has been developed as a patch type to utilize as a molecular sweat sensor. Here, the SERS patch sensor is designed to comprise a sweat-absorbing layer, which is an interface to the human skin, an SERS active layer, and a dermal protecting layer that prevents damage and contaminations. A silk fibroin protein film (SFF) is a basement layer that absorbs aqueous solutions and filtrates molecules larger than the nanopores created in the β-sheet matrix of the SFF. On the SFF layer, a plasmonic silver nanowire (AgNW) layer is formed to enhance the Raman signal of the molecules that penetrated through the SERS patch in a label-free method. A transparent dermal protecting layer (DP) allows laser penetration to the AgNW layer enabling Raman measurement through the SERS patch without its detachment from the surface. The molecular detection capability and time-dependent absorption properties of the SERS patch are investigated, and then, the feasibility of its use as a wearable drug detection sweat sensor is demonstrated using 2-fluoro-methamphetamine (2-FMA) on the human cadaver skin. It is believed that the developed SERS patch can be utilized as various flexible and wearable biosensors for healthcare monitoring.
一种可穿戴式表面增强拉曼散射（SERS）传感器已被开发为一种贴片类型，用作分子汗液传感器。在这里，SERS 贴片传感器设计包括一个吸湿层，作为与人体皮肤的接口，一个 SERS 活性层，和一个防止损伤和污染的真皮保护层。丝素蛋白（SFF）膜是一种基底层，它吸收水溶液并过滤掉大于 SFF 的β-折叠基质中形成的纳米孔的分子。在 SFF 层上形成等离子体银纳米线（AgNW）层，以通过无标记的方法增强穿透 SERS 贴片的分子的拉曼信号。透明的真皮保护层（DP）允许激光穿透到 AgNW 层，从而无需从表面上取下 SERS 贴片即可通过 SERS 进行 Raman 测量。研究了 SERS 贴片的分子检测能力和随时间变化的吸收特性，然后，使用 2-氟甲卡西酮（2-FMA）在人体尸体皮肤上证明了其作为可穿戴式药物检测汗液传感器的可行性。相信开发的 SERS 贴片可以用作各种用于医疗保健监测的灵活的可穿戴式生物传感器。
