Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, Nanjing, 210096, China.
Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, Nanjing, 210096, China.
Biosens Bioelectron. 2024 Dec 1;265:116662. doi: 10.1016/j.bios.2024.116662. Epub 2024 Aug 10.
Sweat biomarkers have the potential to offer valuable clinical insights into an individual's health and disease condition. Current sensors predominantly utilize enzymes and antibodies as biometric components to measure biomarkers present in sweat quantitatively. However, enzymes and antibodies are susceptible to interference by environmental factors, which may affect the performance of the sensor. Herein, we present a wearable microfluidic surface-enhanced Raman scattering (SERS) biosensor that enables the non-invasive and label-free detection of biomarkers in sweat. Concretely, we developed a bimetallic self-assembled anti-opal array structure with uniform hot spots, enhanced the Raman scattering effect, and integrated it into a silk fibroin-based sensing patch. Utilizing a silk fibroin substrate in the wearable SERS sensor imparts desirable properties such as softness, breathability, and biocompatibility, which enables the sensor to establish close contact with the skin without causing chemical or physical irritation. In addition, introducing microfluidic channels enables the controlled and high temporal resolution management of sweat, facilitating more efficient sweat collection. The proposed label-free SERS sensor can offer chemical 'fingerprint' information, enabling the identification of sweat analytes. As an illustration of the feasibility, we have effectively monitored the creatinine and uric acid levels in sweat. This study presents a versatile and highly sensitive approach for the simultaneous detection of biomarkers in human sweat, showcasing significant potential for application in point-of-care monitoring.
汗液生物标志物有可能为个体的健康和疾病状况提供有价值的临床见解。目前的传感器主要使用酶和抗体作为生物识别组件来定量测量汗液中的生物标志物。然而，酶和抗体容易受到环境因素的干扰，这可能会影响传感器的性能。在此，我们提出了一种可穿戴微流控表面增强拉曼散射（SERS）生物传感器，能够非侵入式、无需标记地检测汗液中的生物标志物。具体来说，我们开发了一种具有均匀热点的双金属自组装反蛋白石阵列结构，增强了拉曼散射效应，并将其集成到丝素蛋白基传感片中。在可穿戴 SERS 传感器中使用丝素蛋白基质赋予了其柔软、透气和生物相容性等理想特性，使传感器能够与皮肤紧密接触，而不会引起化学或物理刺激。此外，引入微流道可以实现对汗液的控制和高时间分辨率管理，从而更有效地收集汗液。所提出的无标记 SERS 传感器可以提供化学“指纹”信息，从而能够识别汗液分析物。作为可行性的说明，我们已经有效地监测了汗液中的肌酐和尿酸水平。这项研究提出了一种用于同时检测人体汗液中生物标志物的多功能和高灵敏度方法，展示了在即时护理监测方面的巨大应用潜力。
