Department of Emergency Medicine, Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing, 100730, China.
J Nanosci Nanotechnol. 2021 Mar 1;21(3):1419-1429. doi: 10.1166/jnn.2021.19031.
The research aims to explore the preparation and physicochemical properties of cattle encephalon glycoside and ignotin (CEGI) sustained-release drug loading nanostructured lipid carriers (NLC) and to study the enhanced therapeutic efficacy of CEGI-NLC in treatment of retinal degenerative diseases, thereby providing an objective basis and guidance for clinical medication. The results showed that the particle size of drug-loaded microspheres was 333.2 nm and the potential was 24.9 MV, with an entrapment efficiency of (79±5.6)% and a drug loading efficiency of (7.76±2.3)%. The microspheres were spherical and evenly dispersed. The observations showed that drug-loaded microspheres had specific external sustained release characteristics compared with those of CEGI alone. CEGI-NLC can promote the activity of retinal cells. From the 7th to 28th day, the number of cell layers in the outer and inner nuclear layer retina decreased gradually. Electron microscopy observation showed that the apoptotic nuclear was deformed and the internal segment and cilia were broken down. After 14 and 28 days of CEGI-NLC treatment, the number of layers in the retina was thicker and the number of apoptotic cells was lower than those in the control group. The studies proved that CEGI-NLC has a positive effect on promoting the growth and the development of retinal cells as well as alleviating the process of retinal degeneration in RDS mice.
本研究旨在探索牛脑苷脂和尼可刹米（CEGI）缓释载药纳米结构脂质载体（NLC）的制备及理化性质，并研究 CEGI-NLC 治疗视网膜退行性疾病的增效作用，为临床用药提供客观依据和指导。结果表明，载药微球的粒径为 333.2nm，电位为 24.9MV，包封率为（79±5.6）%，载药率为（7.76±2.3）%。微球呈球形，分散均匀。观察表明，与单独使用 CEGI 相比，载药微球具有特定的外部持续释放特性。CEGI-NLC 可促进视网膜细胞的活性。从第 7 天到第 28 天，外核层和内核层视网膜的细胞层数逐渐减少。电镜观察显示，凋亡核变形，内部节段和纤毛断裂。经 CEGI-NLC 治疗 14 天和 28 天后，视网膜层较厚，凋亡细胞数量低于对照组。研究证明，CEGI-NLC 对促进视网膜细胞的生长和发育以及缓解 RDS 小鼠视网膜退行性变过程具有积极作用。
