College of Chemistry, Jilin University, Changchun, 130021, Jilin, China.
Key Laboratory of Lymphatic Surgery Jilin Province, Engineering Laboratory of Lymphatic Surgery Jilin Province, China-Japan Union Hospital of Jilin University, Changchun, 130031, Jilin, China.
Anal Bioanal Chem. 2021 Feb;413(4):1137-1148. doi: 10.1007/s00216-020-03077-7. Epub 2021 Jan 6.
By connecting 1,8-naphthalimide and indole sulfonate, a ratio fluorescent probe capable of differential detection of hydrogen sulfite and hypochlorite was synthesized for the first time. It was able to achieve the qualitative detection of HSO and ClO with high sensitivity and selectivity, respectively. It provides a multi-purpose probe and is based on different emission channels without mutual interference. The probe has the advantages of larger Stokes shift (ClO: 115 nm, HSO: 88 nm), longer λ (ClO: 515 nm, HSO: 548 nm) and better water solubility (DMF/PBS = 1:99, v/v). In addition, the probe is a ratio fluorescence probe, which can detect fluorescence intensity with two different emission waves. It provides internal self-calibration, reduces interference from the background and increases detection accuracy. In vitro cytotoxicity and imaging experiments show that the probe can effectively perform the detection of exogenous HSO and ClO in cells. It can also achieve the detection of HSO and ClO in the plasma environment. Because the probe can detect endogenous ClO, it also has a good prospect for biological application in identifying tumor cells. Graphical abstract.
首次通过连接 1,8-萘二甲酰亚胺和吲哚磺酸盐，合成了一种能够分别对亚硫酸氢根和次氯酸根进行差异检测的比率荧光探针。它能够分别实现对 HSO 和 ClO 的高灵敏度和选择性的定性检测。它提供了一种多用途探针，基于不同的发射通道，没有相互干扰。该探针具有较大的斯托克斯位移（ClO：115nm，HSO：88nm）、较长的 λ（ClO：515nm，HSO：548nm）和更好的水溶性（DMF/PBS=1:99，v/v）。此外，该探针是一种比率荧光探针，可通过两种不同的发射波检测荧光强度。它提供了内部自校准，减少了背景干扰，提高了检测精度。体外细胞毒性和成像实验表明，该探针可以有效地在细胞中检测外源性 HSO 和 ClO。它还可以在血浆环境中检测 HSO 和 ClO。由于该探针可以检测内源性 ClO，因此它在识别肿瘤细胞方面的生物应用也具有很好的前景。
