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近红外荧光染料用于 HSO 的比色和比率荧光检测。

Colorimetric and Ratiometric Fluorescence Detection of HSO With a NIR Fluorescent Dye.

机构信息

Green Catalysis Center and College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China.

Beijing Key Laboratory of Clothing Materials R & D and Assessment, Beijing Engineering Research Center of Textile, Beijing Institute of Fashion Technology, NanofiberBeijing, 100029, China.

出版信息

J Fluoresc. 2021 Sep;31(5):1567-1574. doi: 10.1007/s10895-021-02794-1. Epub 2021 Aug 2.

Abstract

Bisulfite (HSO) has been widely used in food and industry, which has brought convenience to human life, but also seriously endangered human health. In this work, the probe PBI was designed and synthesized to detect bisulfite (HSO) through nucleophilic addition reaction. The probe PBI showed a selective reaction to HSO and can quantitatively detect HSO. At the same time, the color of the probe PBI changed significantly, which provided a simple method for the naked eye to identify HSO. Finally, it was successfully applied to the fluorescence imaging of HSO in living cells.

摘要

亚硫酸氢盐(HSO)在食品和工业中得到了广泛的应用,给人类生活带来了便利,但也严重威胁到人类健康。在这项工作中,设计并合成了探针 PBI 通过亲核加成反应来检测亚硫酸氢盐(HSO)。探针 PBI 对 HSO 表现出选择性反应,并可以定量检测 HSO。同时,探针 PBI 的颜色发生了明显变化,为肉眼识别 HSO 提供了一种简单的方法。最后,成功地将其应用于活细胞中亚硫酸氢盐的荧光成像。

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