State Key Laboratory of New Optical Functional Materials, Anyang Normal University, Anyang, Henan, 455000, China; Department of Chemistry, Clemson University, Clemson, 29634, South Carolina, USA.
State Key Laboratory of New Optical Functional Materials, Anyang Normal University, Anyang, Henan, 455000, China.
Anal Chim Acta. 2024 Sep 1;1320:342993. doi: 10.1016/j.aca.2024.342993. Epub 2024 Jul 19.
Bisulfite (HSO) serves as a bleaching agent, antioxidant, antimicrobial, and regulator of enzymatic reactions in biosystem. However, abnormal levels of bisulfite can be detrimental to health. Hypochlorous acid (HOCl), which acts as bioactive small molecules, is crucial for maintaining normal biological functions in living organisms. Disruption of its equilibrium can lead to oxidative stress and various diseases. Therefore, it's essential to monitor the fluctuations of HOCl and HSO at cellular and in vivo levels to study their physiological and pathological functions.
This study constructed a novel NIR bifunctional colorimetric fluorescent probe using thienocoumarin-indanedione structures to identify hypochlorite (ClO) and bisulfite (HSO). By using CSO-IO to recognize HSO and HOCl, two distinct products were generated, displaying green and blue fluorescence, respectively. This property effectively allows for the simultaneous dual-functional detection of HSO (LOD: 113 nM) and HOCl (LOD: 43 nM).
In this work, the biocompatible molecule CSO-IO has been effectively designed to detect HOCl/HSO in living cells and zebrafish. As a result, the dual-functional fluorescent probe has the potential to be utilized as a molecular tool to detect HSO derived compounds and HOCl simultaneously within the complex biological system.
亚硫酸氢盐（HSO）可用作漂白剂、抗氧化剂、抗菌剂和生物体系中酶反应的调节剂。然而，亚硫酸氢盐水平异常可能对健康有害。次氯酸（HOCl）作为生物活性小分子，对维持生物体内正常的生物功能至关重要。其平衡的破坏会导致氧化应激和各种疾病。因此，监测 HOCl 和 HSO 在细胞和体内水平的波动对于研究其生理和病理功能至关重要。
本研究构建了一种新型的近红外双功能比色荧光探针，使用噻吩并香豆素-茚二酮结构来识别次氯酸盐（ClO）和亚硫酸氢盐（HSO）。通过使用 CSO-IO 识别 HSO 和 HOCl，分别产生了绿色和蓝色荧光两种不同的产物。这种特性有效地实现了 HSO（LOD：113 nM）和 HOCl（LOD：43 nM）的同时双重功能检测。
在这项工作中，设计了生物相容性分子 CSO-IO 以有效检测活细胞和斑马鱼中的 HOCl/HSO。因此，这种双功能荧光探针有可能成为一种分子工具，用于在复杂的生物体系中同时检测 HSO 衍生化合物和 HOCl。
