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根瘤特异性基因调控：异源共生系统中大豆根瘤蛋白N23基因启动子的分析

Root nodule specific gene regulation: analysis of the soybean nodulin N23 gene promoter in heterologous symbiotic systems.

作者信息

Jørgensen J E, Stougaard J, Marcker A, Marcker K A

机构信息

Department of Molecular Biology and Plant Physiology, University of Aarhus, Denmark.

出版信息

Nucleic Acids Res. 1988 Jan 11;16(1):39-50. doi: 10.1093/nar/16.1.39.

DOI:10.1093/nar/16.1.39
PMID:3340542
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334611/
Abstract

The nodulin N23 gene promoter was analysed in transgenic plants using the chloramphenicol acetyltransferase (CAT) coding sequence as a reporter. A 5' flanking region of less than 1 kb was sufficient for the organ-specific expression of a chimeric N23-CAT-3'lbc3 gene in root nodules formed on Lotus corniculatus and Trifolium repens after infection by their respective Rhizobium symbionts. Expression was regulated at the level of RNA in both species of transgenic plants. Promoter deletion analysis defined the 5' region required for high level expression and delimited two putative regulatory sequences involved in positive control of the N23 gene in L. corniculatus.

摘要

利用氯霉素乙酰转移酶（CAT）编码序列作为报告基因，在转基因植物中分析了结节蛋白N23基因启动子。小于1kb的5'侧翼区域足以使嵌合N23-CAT-3'lbc3基因在其各自的根瘤菌共生体感染后在百脉根和白三叶形成的根瘤中进行器官特异性表达。在两种转基因植物中，表达均在RNA水平上受到调控。启动子缺失分析确定了高水平表达所需的5'区域，并界定了两个与百脉根中N23基因的正调控有关的推定调控序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/099a/334611/a33ffb360dd2/nar00143-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/099a/334611/2550712124f5/nar00143-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/099a/334611/cfab804e85cc/nar00143-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/099a/334611/3f8001c9bcff/nar00143-0056-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/099a/334611/a33ffb360dd2/nar00143-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/099a/334611/2550712124f5/nar00143-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/099a/334611/cfab804e85cc/nar00143-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/099a/334611/3f8001c9bcff/nar00143-0056-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/099a/334611/a33ffb360dd2/nar00143-0058-a.jpg

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本文引用的文献

1
Organ-specific and light-induced expression of plant genes.植物基因的组织特异性和光诱导表达。
Science. 1986 May 30;232(4754):1106-12. doi: 10.1126/science.232.4754.1106.
2
Sucrose synthase of soybean nodules.大豆根瘤中的蔗糖合酶。
Plant Physiol. 1985 May;78(1):149-54. doi: 10.1104/pp.78.1.149.
3
Enzymes of ureide synthesis in pea and soybean.脲合成酶在豌豆和大豆中的作用。
Plant Cell Rep. 1989 May;8(1):12-5. doi: 10.1007/BF00735768.
4
Regulatory regions and nuclear factors involved in nodule-enhanced expression of a soybean phosphoenolpyruvate carboxylase gene: implications for molecular evolution.参与大豆磷酸烯醇式丙酮酸羧化酶基因根瘤增强表达的调控区域和核因子：对分子进化的启示
Mol Genet Genomics. 2003 May;269(2):163-72. doi: 10.1007/s00438-002-0794-y. Epub 2003 Mar 28.
5
A novel type of DNA-binding protein interacts with a conserved sequence in an early nodulin ENOD12 promoter.一种新型的DNA结合蛋白与早期结瘤素ENOD12启动子中的保守序列相互作用。
Plant Mol Biol. 1996 Dec;32(5):809-21. doi: 10.1007/BF00020479.
6
Minimal enhancer elements of the leghemoglobin lba and lbc3 gene promoters from Glycine max L. have different properties.来自大豆（Glycine max L.）的豆血红蛋白lba和lbc3基因启动子的最小增强子元件具有不同特性。
Plant Mol Biol. 1993 Sep;22(6):945-56. doi: 10.1007/BF00028968.
7
A protein binding AT-rich sequence in the soybean leghemoglobin c3 promoter is a general cis element that requires proximal DNA elements to stimulate transcription.大豆根瘤血红蛋白c3启动子中与富含AT序列结合的一种蛋白质是一种通用顺式元件，它需要近端DNA元件来刺激转录。
Plant Cell. 1994 May;6(5):659-68. doi: 10.1105/tpc.6.5.659.
8
Analysis of the lupin Nodulin-45 promoter: conserved regulatory sequences are important for promoter activity.羽扇豆根瘤球蛋白-45启动子分析：保守调控序列对启动子活性至关重要。
Plant Mol Biol. 1995 Feb;27(3):457-66. doi: 10.1007/BF00019313.
9
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10
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Plant Physiol. 1983 May;72(1):56-9. doi: 10.1104/pp.72.1.56.
4
Expression of a complete soybean leghemoglobin gene in root nodules of transgenic Lotus corniculatus.在转基因 Lotus corniculatus 的根瘤中表达完整的大豆根瘤血红蛋白基因。
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5754-7. doi: 10.1073/pnas.84.16.5754.
5
Light-induced expression of the chimeric chalcone synthase-NPTII gene in tobacco cells.光诱导嵌合查尔酮合酶 - 新霉素磷酸转移酶II基因在烟草细胞中的表达。
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6
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7
Transcription of the soybean leghemoglobin genes during nodule development.在根瘤发育过程中大豆血红蛋白基因的转录。
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8
Nodulin-35: a subunit of specific uricase (uricase II) induced and localized in the uninfected cells of soybean nodules.类结蛋白-35：尿酸酶（尿酸酶 II）的一个亚基，在未感染的大豆根瘤细胞中诱导和定位。
EMBO J. 1983;2(12):2333-9. doi: 10.1002/j.1460-2075.1983.tb01743.x.
9
Promoter analysis of a soybean nuclear gene coding for nodulin-23, a nodule-specific polypeptide involved in symbiosis with Rhizobium.对编码根瘤菌素-23的大豆核基因的启动子分析，根瘤菌素-23是一种与根瘤菌共生相关的根瘤特异性多肽。
EMBO J. 1985 Oct;4(10):2431-8. doi: 10.1002/j.1460-2075.1985.tb03952.x.
10
Identification of "nodule-specific" host proteins (nodoulins) involved in the development of rhizobium-legume symbiosis.鉴定参与根瘤菌 - 豆科植物共生关系形成的“结节特异性”宿主蛋白（结节蛋白）。
Cell. 1980 May;20(1):153-63. doi: 10.1016/0092-8674(80)90243-3.