大豆根瘤素-23基因的一级结构以及根瘤素和豆血红蛋白基因5'侧翼区域中的潜在调控元件。
Primary structure of the soybean nodulin-23 gene and potential regulatory elements in the 5'-flanking regions of nodulin and leghemoglobin genes.
作者信息
Mauro V P, Nguyen T, Katinakis P, Verma D P
出版信息
Nucleic Acids Res. 1985 Jan 11;13(1):239-49. doi: 10.1093/nar/13.1.239.
Abstract
The nodulin-23 gene of soybean is one of the most abundantly transcribed genes induced during symbiosis with Rhizobium. Using a plasmid (pNod25) from a nodule cDNA library, we have isolated the nodulin-23 gene from a soybean genomic library. Nucleotide sequence analysis of the cDNA and of the genomic clone indicated that the coding region of this gene is 669 bp long and is interrupted by a single intron of about 530 bp. The deduced protein sequence suggests that nodulin-23 may have a signal sequence. The 5'-flanking sequence of two other nodulin genes, nodulin-24 encoding for a membrane polypeptide and one of the leghemoglobin genes (LbC3), were obtained. Comparison of these sequences revealed three conserved regions, one of which, an octanucleotide (GTTTCCCT), has 100% homology. The conserved sequences are arranged in a unique fashion and have a spatial organization with respect to order and position, which may suggest a potential regulatory role in controlling the expression of nodulin and leghemoglobin genes during symbiosis.
摘要
大豆的根瘤菌素-23基因是在与根瘤菌共生期间诱导转录最丰富的基因之一。利用来自根瘤cDNA文库的质粒（pNod25），我们从大豆基因组文库中分离出了根瘤菌素-23基因。对cDNA和基因组克隆的核苷酸序列分析表明，该基因的编码区长度为669 bp，被一个约530 bp的单一内含子打断。推导的蛋白质序列表明根瘤菌素-23可能有一个信号序列。获得了另外两个根瘤菌素基因的5'侧翼序列，一个是编码膜多肽的根瘤菌素-24，另一个是豆血红蛋白基因之一（LbC3）。对这些序列的比较揭示了三个保守区域，其中一个八核苷酸（GTTTCCCT）具有100%的同源性。保守序列以独特的方式排列，并且在顺序和位置方面具有空间组织，这可能表明在共生期间控制根瘤菌素和豆血红蛋白基因表达方面具有潜在的调节作用。
相似文献
1
Primary structure of the soybean nodulin-23 gene and potential regulatory elements in the 5'-flanking regions of nodulin and leghemoglobin genes.大豆根瘤素-23基因的一级结构以及根瘤素和豆血红蛋白基因5'侧翼区域中的潜在调控元件。
Nucleic Acids Res. 1985 Jan 11;13(1):239-49. doi: 10.1093/nar/13.1.239.
2
Characterization of a novel nodulin gene in soybean that shares sequence similarity to the gene for nodulin-24.大豆中一个与根瘤菌素-24基因具有序列相似性的新型根瘤菌素基因的特性分析。
Plant Mol Biol. 1990 Dec;15(6):835-49. doi: 10.1007/BF00039424.
3
Interdependence and nodule specificity of cis-acting regulatory elements in the soybean leghemoglobin lbc3 and N23 gene promoters.大豆豆血红蛋白lbc3和N23基因启动子中顺式作用调控元件的相互依赖性和结节特异性
Mol Gen Genet. 1990 Feb;220(3):353-60. doi: 10.1007/BF00391738.
4
Site-directed mutagenesis of the organ-specific element in the soybean leghemoglobin lbc3 gene promoter.大豆豆血红蛋白lbc3基因启动子中器官特异性元件的定点诱变。
Plant J. 1993 Sep;4(3):577-80. doi: 10.1046/j.1365-313x.1993.04030577.x.
5
Characterization and genomic organization of a highly expressed late nodulin gene subfamily in soybeans.
Mol Gen Genet. 1991 Oct;229(3):445-52. doi: 10.1007/BF00267468.
6
5' flanking sequence of the soybean leghemoglobin lbc3 gene.大豆豆血红蛋白lbc3基因的5'侧翼序列。
Nucleic Acids Res. 1989 Jun 12;17(11):4383. doi: 10.1093/nar/17.11.4383.
7
Soybean leghemoglobin gene family: normal, pseudo, and truncated genes.大豆豆血红蛋白基因家族：正常基因、假基因和截短基因。
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4055-9. doi: 10.1073/pnas.79.13.4055.
8
The primary structures of two leghemoglobin genes from soybean.来自大豆的两个豆血红蛋白基因的一级结构。
Nucleic Acids Res. 1982 Jan 22;10(2):689-701. doi: 10.1093/nar/10.2.689.
9
Chromosomal arrangement of leghemoglobin genes in soybean.大豆中豆血红蛋白基因的染色体排列
Nucleic Acids Res. 1983 Aug 25;11(16):5541-53. doi: 10.1093/nar/11.16.5541.
10
The nucleotide sequences of two leghemoglobin genes from soybean.来自大豆的两个豆血红蛋白基因的核苷酸序列。
Nucleic Acids Res. 1982 Jun 11;10(11):3487-94. doi: 10.1093/nar/10.11.3487.
引用本文的文献
1
Nodulins and nodulin genes of Glycine max.大豆球蛋白和大豆球蛋白基因。
Plant Mol Biol. 1986 Jan;7(1):51-61. doi: 10.1007/BF00020131.
2
A block in the endocytosis of Rhizobium allows cellular differentiation in nodules but affects the expression of some peribacteroid membrane nodulins.根瘤菌内吞作用受阻会影响根瘤细胞分化，但不影响类菌体周膜豆血红蛋白基因的表达。
Plant Mol Biol. 1987 May;9(3):185-96. doi: 10.1007/BF00166455.
3
Primary structure of the soybean nodulin-35 gene encoding uricase II localized in the peroxisomes of uninfected cells of nodules.大豆尿囊素 35 基因编码尿酸酶 II 的一级结构，该酶定位于根瘤细胞的过氧化物酶体中。
Proc Natl Acad Sci U S A. 1985 Aug;82(15):5040-4. doi: 10.1073/pnas.82.15.5040.
4
Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris.菜豆根瘤、根系和叶片中谷氨酰胺合成酶基因的一级结构和差异表达。
EMBO J. 1986 Jul;5(7):1429-35. doi: 10.1002/j.1460-2075.1986.tb04379.x.
5
Promoter analysis of a soybean nuclear gene coding for nodulin-23, a nodule-specific polypeptide involved in symbiosis with Rhizobium.对编码根瘤菌素-23的大豆核基因的启动子分析，根瘤菌素-23是一种与根瘤菌共生相关的根瘤特异性多肽。
EMBO J. 1985 Oct;4(10):2431-8. doi: 10.1002/j.1460-2075.1985.tb03952.x.
6
Multiple transcripts of a gene for a leucine-rich repeat receptor kinase from morning glory (Ipomoea nil) originate from different TATA boxes in a tissue-specific manner.来自牵牛花（裂叶牵牛）的富含亮氨酸重复序列受体激酶基因的多个转录本以组织特异性方式源自不同的TATA框。
Mol Genet Genomics. 2004 Jul;271(6):752-60. doi: 10.1007/s00438-004-1031-7. Epub 2004 Jun 19.
7
Soybean nodulin-26 gene encoding a channel protein is expressed only in the infected cells of nodules and is regulated differently in roots of homologous and heterologous plants.编码一种通道蛋白的大豆根瘤球蛋白-26基因仅在根瘤的受感染细胞中表达，且在同源和异源植物的根中受到不同的调控。
Plant Cell. 1993 Jul;5(7):781-94. doi: 10.1105/tpc.5.7.781.
8
Isolation and characterization of novel nodulin cDNAs representing genes expressed at early stages of soybean nodule development.新型根瘤素cDNA的分离与鉴定，这些cDNA代表在大豆根瘤发育早期表达的基因。
Mol Gen Genet. 1993 Apr;238(1-2):106-19. doi: 10.1007/BF00279537.
9
Characterization and gene expression of nodulin Npv30 from common bean.菜豆根瘤蛋白Npv30的特性及基因表达
Plant Physiol. 1995 Oct;109(2):363-70. doi: 10.1104/pp.109.2.363.
10
Nucleotide sequence of a B1 hordein gene and the identification of possible upstream regulatory elements in endosperm storage protein genes from barley, wheat and maize.大麦、小麦和玉米胚乳贮藏蛋白基因中一个B1醇溶蛋白基因的核苷酸序列及可能的上游调控元件的鉴定。
Nucleic Acids Res. 1985 Oct 25;13(20):7327-39. doi: 10.1093/nar/13.20.7327.
本文引用的文献
1
A nodule-specific plant protein (nodulin-35) from soybean.一种来自大豆的结节特异性植物蛋白（结节蛋白-35）。
Science. 1979 Jul 13;205(4402):190-3. doi: 10.1126/science.205.4402.190.
2
Soybean nodulin genes: Analysis of cDNA clones reveals several major tissue-specific sequences in nitrogen-fixing root nodules.大豆结瘤素基因：cDNA 克隆分析揭示固氮根瘤中几种主要的组织特异性序列。
Proc Natl Acad Sci U S A. 1983 May;80(9):2594-8. doi: 10.1073/pnas.80.9.2594.
3
Nodulin-35: a subunit of specific uricase (uricase II) induced and localized in the uninfected cells of soybean nodules.类结蛋白-35：尿酸酶（尿酸酶 II）的一个亚基，在未感染的大豆根瘤细胞中诱导和定位。
EMBO J. 1983;2(12):2333-9. doi: 10.1002/j.1460-2075.1983.tb01743.x.
4
Nodule-specific host proteins in effective and ineffective root nodules of Pisum sativum.豌豆中有效和无效根瘤的结节特异性宿主蛋白。
EMBO J. 1983;2(6):961-6. doi: 10.1002/j.1460-2075.1983.tb01528.x.
5
Structure, organization and evolution of developmentally regulated chorion genes in a silkmoth.家蚕中发育调控型绒毛膜基因的结构、组织与进化
Cell. 1980 Dec;22(3):855-67. doi: 10.1016/0092-8674(80)90562-0.
6
Identification of "nodule-specific" host proteins (nodoulins) involved in the development of rhizobium-legume symbiosis.鉴定参与根瘤菌 - 豆科植物共生关系形成的“结节特异性”宿主蛋白（结节蛋白）。
Cell. 1980 May;20(1):153-63. doi: 10.1016/0092-8674(80)90243-3.
7
Multiple mRNAs are generated from the chicken lysozyme gene.鸡溶菌酶基因可产生多种信使核糖核酸。
Cell. 1981 Sep;25(3):743-52. doi: 10.1016/0092-8674(81)90182-3.
8
A simple method for displaying the hydropathic character of a protein.一种展示蛋白质亲水性特征的简单方法。
J Mol Biol. 1982 May 5;157(1):105-32. doi: 10.1016/0022-2836(82)90515-0.
9
The DNA sequence of the 5' flanking region of the human beta-globin gene: evolutionary conservation and polymorphic differences.人类β-珠蛋白基因5'侧翼区的DNA序列：进化保守性与多态性差异
Nucleic Acids Res. 1982 Mar 25;10(6):2109-20. doi: 10.1093/nar/10.6.2109.
10
Primary structure and evolution of rat growth hormone gene.大鼠生长激素基因的一级结构与进化
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4867-71. doi: 10.1073/pnas.78.8.4867.
Zotero 插件