Gutierrez Elena, Burdiles Patricio A, Quero Franck, Palma Patricia, Olate-Moya Felipe, Palza Humberto
Laboratorio de Microbiología Oral, Área Péptidos y Compuestos Antimicrobianos, Facultad de Odontología, Universidad de Chile, Olivos 943, 8380544 Independencia, Chile.
ACS Biomater Sci Eng. 2019 Nov 11;5(11):6290-6299. doi: 10.1021/acsbiomaterials.9b01048. Epub 2019 Oct 23.
Novel antimicrobial 3D-printed alginate/bacterial-cellulose hydrogels with in situ-synthesized copper nanostructures were developed having improved printability. Prior to 3D printing, two methods were tested for the development of the alginate hydrogels: (a) ionic cross-linking with calcium ions followed by ion exchange with copper ions (method A) and (b) ionic cross-linking with copper ions (method B). A solution containing sodium borohydride, used as a reducing agent, was subsequently added to the hydrogels, producing in situ clusters of copper nanoparticles embedded in the alginate hydrogel matrix. The method used and concentrations of copper and the reducing agent were found to affect the stability of the alginate/copper hydrogels, with method A producing more stable materials. By increasing the alginate concentration from 1 to 4 wt % and by using method A, alginate/bacterial-cellulose/copper hydrogel structures were 3D-printed having excellent printability as compared with pure alginate hydrogels. It is noteworthy that after reduction with sodium borohydride, the 3D structures presented antimicrobial behavior against and strains. Our results introduce a simple route for the production of alginate/cellulose inks with improved behavior toward antimicrobial 3D-printed materials.
开发了具有原位合成铜纳米结构的新型抗菌3D打印藻酸盐/细菌纤维素水凝胶，其具有改进的可打印性。在3D打印之前，测试了两种制备藻酸盐水凝胶的方法：（a）用钙离子进行离子交联，然后与铜离子进行离子交换（方法A）和（b）用铜离子进行离子交联（方法B）。随后将用作还原剂的含硼氢化钠的溶液添加到水凝胶中，在藻酸盐水凝胶基质中产生原位铜纳米颗粒簇。发现所使用的方法以及铜和还原剂的浓度会影响藻酸盐/铜水凝胶的稳定性，方法A产生的材料更稳定。通过将藻酸盐浓度从1 wt％增加到4 wt％并使用方法A，与纯藻酸盐水凝胶相比，3D打印出的藻酸盐/细菌纤维素/铜水凝胶结构具有优异的可打印性。值得注意的是，在用硼氢化钠还原后，3D结构对 和 菌株呈现出抗菌行为。我们的结果为生产对抗菌3D打印材料具有改进性能的藻酸盐/纤维素墨水引入了一条简单途径。
