Choi Moonhyun, Park Sohyeon, Park Kyungtae, Jeong Hyejoong, Hong Jinkee
Department of Chemical and Biomolecular Engineering, College of Engineering, Yonsei University 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
ACS Biomater Sci Eng. 2019 Mar 11;5(3):1378-1383. doi: 10.1021/acsbiomaterials.9b00016. Epub 2019 Feb 13.
Nitric oxide (NO) participates in various physiological and pathophysiological processes, for example, as a cell messenger and as an antimicrobial agent of the cell-mediated immune response. The development of NO-releasing materials to carry and deliver NO for biomedical applications has gained immense attention. NO-releasing perfluorooctane (PFO) microemulsion (ME) has been prepared using a simple and time-saving method. Perfluorocarbon (PFC) liquids are halogen-substituted carbon nonpolar oils with enhanced NO gas dissolution capacity. The solubility of NO in PFC liquids is higher than that in water-based fluids. Liquid-gas solubility is governed by Henry's Law. The cytotoxicity of the NO-unloaded and NO-loaded PFO MEs toward human dermal fibroblast (HDF) was evaluated. The results showed that the NO-loaded PFO ME was highly biocompatible. On the other hand, at high concentrations the NO-releasing PFO ME accelerated the bacteria () death unlike the NO-unloaded PFO ME. Hence, NO-releasing PFO MEs are suitable for biomedical applications such as wound healing and antibacterial agents.
一氧化氮（NO）参与各种生理和病理生理过程，例如，作为细胞信使以及作为细胞介导免疫反应的抗菌剂。用于生物医学应用的携带和递送NO的NO释放材料的开发已引起广泛关注。已使用一种简单且省时的方法制备了释放NO的全氟辛烷（PFO）微乳液（ME）。全氟化碳（PFC）液体是具有增强的NO气体溶解能力的卤素取代的碳非极性油。NO在PFC液体中的溶解度高于在水基流体中的溶解度。液-气溶解度受亨利定律支配。评估了未负载NO和负载NO的PFO微乳液对人皮肤成纤维细胞（HDF）的细胞毒性。结果表明，负载NO的PFO微乳液具有高度生物相容性。另一方面，与未负载NO的PFO微乳液不同，在高浓度下，释放NO的PFO微乳液加速了细菌（）死亡。因此，释放NO的PFO微乳液适用于诸如伤口愈合和抗菌剂等生物医学应用。
