Lusk Joel F, Miranda Christopher, Howell Madeleine, Chrest Matthew, Eshima Jarrett, Smith Barbara S
School of Biological and Health Systems Engineering, Arizona State University, 550 E. Orange Street, Tempe, Arizona 85281, United States.
ACS Biomater Sci Eng. 2019 Mar 11;5(3):1553-1560. doi: 10.1021/acsbiomaterials.8b01217. Epub 2019 Feb 21.
The development of cell-specific photoacoustic (PA) contrast agents within systems of fluidic flow provides opportunities for the accurate detection of early stage cancer metastasis. Despite the promise of exogenous contrast agents for use in clinical settings, applications are currently limited by both material biocompatibility and target specificity. In this study, folic acid functionalized copper sulfide nanoparticles (FA-CuS NPs) are synthesized to enable ovarian-cancer-specific binding and PA detection in a custom flow system. Folate receptors, known to be overexpressed on the surface of ovarian cancer cells, have remained an ideal candidate for specific targeting through functionalization on nanoparticles and other contrast agents. In combination with copper sulfide nanoparticles' strong absorbance in the near-infrared (NIR), these FA-CuS NPs are an ideal contrast agent capable of being detected by photoacoustic flow cytometry. For the first time, this study shows a potential PA contrast agent to accurately identify ovarian circulating tumor cells in flow.
在流体流动系统中开发细胞特异性光声（PA）造影剂为早期癌症转移的精确检测提供了机会。尽管外源性造影剂有望用于临床，但目前其应用受到材料生物相容性和靶点特异性的限制。在本研究中，合成了叶酸功能化硫化铜纳米颗粒（FA-CuS NPs），以实现定制流动系统中卵巢癌特异性结合和PA检测。叶酸受体在卵巢癌细胞表面过表达，一直是通过纳米颗粒和其他造影剂功能化进行特异性靶向的理想候选者。结合硫化铜纳米颗粒在近红外（NIR）区域的强吸收，这些FA-CuS NPs是一种理想的造影剂，能够通过光声流式细胞术进行检测。本研究首次展示了一种潜在的PA造影剂，可在流动中准确识别卵巢循环肿瘤细胞。
