Foroutan Farzad, McGuire Jamie, Gupta Priyanka, Nikolaou Athanasios, Kyffin Benjamin A, Kelly Nicole L, Hanna John V, Gutierrez-Merino Jorge, Knowles Jonathan C, Baek Song-Yi, Velliou Eirini, Carta Daniela
Department of Physics, University of Warwick, Coventry CV4 7AL, United Kingdom.
Division of Biomaterials and Tissue Engineering, Eastman Dental Institute, University College London, 256 Gray's Inn Road, London WC1X 8LD, United Kingdom.
ACS Biomater Sci Eng. 2019 Nov 11;5(11):6054-6062. doi: 10.1021/acsbiomaterials.9b01291. Epub 2019 Oct 25.
Calcium phosphate glasses are a promising new generation of biomaterials that can simultaneously induce tissue regeneration and controlled release of therapeutic molecules. In this work, novel calcium phosphate glasses containing 0, 2, 4, and 6 mol % Cu were synthesized via room temperature precipitation reaction in aqueous solution. The effect of Cu addition on the glass properties and structure was investigated using thermal analysis, P solid-state MAS NMR, Raman spectroscopy, and X-ray diffraction. All glasses crystallize at temperature >500 °C and are mainly formed by Q groups. The release of P, Ca, and Cu in solution over time was monitored via inductively coupled plasma-optical emission spectroscopy. It was found that with increasing Cu content, the amount of P and Ca released decreases whereas the amount of Cu released increases. The effect of Cu release on the antibacterial activity against , a bacterial strain commonly found in postsurgery infections, has been investigated. The addition of copper has been shown to infer the glasses antibacterial properties. As expected, the antibacterial activity of the glasses increases with increasing Cu content. Cytocompatibility was assessed by seeding human osteoblast-like osteosarcoma cells Saos-2 (HTB85) on the glass particles. A significant increase in cell number was observed in all the glasses investigated. The copper-doped calcium phosphate glasses have proven to be multifunctional, as they combine bone regenerative properties with antibacterial activity. Therefore, they have great potential as antibacterial bioresorbable materials for hard tissue regeneration.
磷酸钙玻璃是新一代很有前景的生物材料，能够同时诱导组织再生并实现治疗分子的控释。在本研究中，通过水溶液中的室温沉淀反应合成了含0、2、4和6摩尔%铜的新型磷酸钙玻璃。使用热分析、磷固态核磁共振、拉曼光谱和X射线衍射研究了铜添加量对玻璃性能和结构的影响。所有玻璃在温度>500°C时结晶，且主要由Q基团构成。通过电感耦合等离子体发射光谱监测溶液中磷、钙和铜随时间的释放情况。结果发现，随着铜含量的增加，磷和钙的释放量减少，而铜的释放量增加。研究了铜释放对针对一种常见于术后感染的细菌菌株的抗菌活性的影响。已证明添加铜可赋予玻璃抗菌性能。正如预期的那样，玻璃的抗菌活性随铜含量的增加而增强。通过将人成骨样骨肉瘤细胞Saos-2（HTB85）接种在玻璃颗粒上来评估细胞相容性。在所研究的所有玻璃中均观察到细胞数量显著增加。掺杂铜的磷酸钙玻璃已被证明具有多功能性，因为它们兼具骨再生特性和抗菌活性。因此，它们作为用于硬组织再生的抗菌生物可吸收材料具有巨大潜力。
