载铜磷酸盐玻璃对致病菌的抗菌和抗生物膜效果。
Antibacterial and Antibiofilm Efficacy of Copper-Doped Phosphate Glass on Pathogenic Bacteria.
机构信息
Preventive and Restorative Dentistry Department, College of Dental Medicine, University of Sharjah, Sharjah P.O. Box 27272, United Arab Emirates.
RIMHS, Sharjah Institute for Medical Research, University of Sharjah, Sharjah P.O. Box 27272, United Arab Emirates.
出版信息
Molecules. 2023 Apr 3;28(7):3179. doi: 10.3390/molecules28073179.
UNLABELLED
This study aimed to investigate the antibacterial [minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC)] and antibiofilm activity [log10 colony forming unit/mL (CFU/mL) and biofilm disruption] of copper-doped phosphate glass (CDPG) against and .
METHODS
the antibacterial activity was determined using microbroth dilution and time-kill assay. The antibiofilm activity was investigated using crystal violet and confocal laser scanning microscopy. Bacteria growing in absence of CDPG were used as controls.
RESULTS
the MIC was ≥125 mg of CPDG/mL; the log10 CFU/mL reduction ranged from 2.66-3.14 to 6.23-9.65 after 4 and 24 h respectively. Generally, no growth was observed after 24 h of treatment with CDPG; the MBC was 250 mg/mL for and while 500 mg/mL for the rest of the bacteria. The highest and lowest antibiofilm activity was observed against and respectively. Three patterns of complete biofilm disruption were seen: (i) large areas with and , (ii) medium-size pockets with and or (iii) small areas with and .
CONCLUSION
CDPG can be potentially used as an antibacterial and an antibiofilm agent against oral biofilm-forming bacteria.
本研究旨在探讨掺铜磷酸盐玻璃（CDPG）对 和 的抗菌（最小抑菌浓度（MIC）和最小杀菌浓度（MBC））和抗生物膜活性（log10 菌落形成单位/mL（CFU/mL）和生物膜破坏）。
方法
采用微量肉汤稀释法和时间杀伤试验测定抗菌活性。采用结晶紫和共聚焦激光扫描显微镜研究抗生物膜活性。未添加 CDPG 的细菌生长作为对照。
结果
MIC 为≥125 mg CPDG/mL；4 小时和 24 小时后，log10 CFU/mL 减少分别为 2.66-3.14 至 6.23-9.65。通常，用 CDPG 处理 24 小时后观察不到生长；MBC 分别为 250 mg/mL 和 ，而其余细菌为 500 mg/mL。对 和 的抗生物膜活性最高和最低。观察到三种完全破坏生物膜的模式：（i） 和 有大片区域，（ii） 和 或 有中等大小的口袋，（iii） 和 有小区域。
结论
CDPG 可作为一种抗菌和抗生物膜剂，用于抑制口腔生物膜形成细菌。
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