Yuan Hongyuan, Yang Yong, Xue Wei, Liu Zonghua
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, West Huangpu Road 601, Guangzhou 510632, China.
ACS Biomater Sci Eng. 2019 Feb 11;5(2):644-653. doi: 10.1021/acsbiomaterials.8b00945. Epub 2018 Dec 12.
As a potential method for tumor treatment, tumor vaccine immunization induces a tumor-specific cellular immune response via immunization with tumor antigens. The delivery of exogenous antigen proteins into the cytoplasm of antigen-presenting cells is well known to induce an intensive cellular immune response for tumor treatment. In this work, we fluorinated a redox-responsive hyperbranched poly(amidoamine) (HPAA) with heptafluorobutyric anhydride to prepare a fluorinated HPAA (HPAA-F7) for use as a vaccine delivery system for antitumor therapy. The immunization results show that HPAA-F7 as a vaccine carrier could effectively promote the intracellular uptake and cytoplasmatic delivery of antigen proteins and induce potent antitumor cellular immunity. The novel vaccine carrier HPAA-F7 could be further developed for antitumor immunotherapy.
作为一种潜在的肿瘤治疗方法，肿瘤疫苗免疫通过用肿瘤抗原进行免疫来诱导肿瘤特异性细胞免疫反应。众所周知，将外源性抗原蛋白递送至抗原呈递细胞的细胞质中可诱导强烈的细胞免疫反应以用于肿瘤治疗。在这项工作中，我们用七氟丁酸酐对氧化还原响应性超支化聚（酰胺胺）（HPAA）进行氟化，以制备氟化HPAA（HPAA-F7）用作抗肿瘤治疗的疫苗递送系统。免疫结果表明，HPAA-F7作为疫苗载体可有效促进抗原蛋白的细胞内摄取和细胞质递送，并诱导强大的抗肿瘤细胞免疫。新型疫苗载体HPAA-F7可进一步开发用于抗肿瘤免疫治疗。
