Kosik-Kozioł Alicja, Graham Elizabeth, Jaroszewicz Jakub, Chlanda Adrian, Kumar P T Sudheesh, Ivanovski Saso, Święszkowski Wojciech, Vaquette Cedryck
Faculty of Materials Science and Engineering, Warsaw University of Technology (WUT), 02-507 Warsaw, Poland.
Queensland University of Technology (QUT), Brisbane, Queensland 4001, Australia.
ACS Biomater Sci Eng. 2019 Jan 14;5(1):318-328. doi: 10.1021/acsbiomaterials.8b01018. Epub 2018 Dec 10.
One promising strategy to reconstruct bone defects relies on 3D printed porous structures. In spite of several studies having been carried out to fabricate controlled, interconnected porous constructs, the control over surface features at, or below, the microscopic scale remains elusive for 3D polymeric scaffolds. In this study, we developed and refined a methodology which can be applied to homogeneously and reproducibly modify the surface of polymeric 3D printed scaffolds. We have demonstrated that the combination of a polymer solvent and the utilization of ultrasound was essential for achieving appropriate surface modification without damaging the structural integrity of the construct. The modification created on the scaffold profoundly affected the macroscopic and microscopic properties of the scaffold with an increased roughness, greater surface area, and reduced hydrophobicity. Furthermore, to assess the performance of such materials in bone tissue engineering, human mesenchymal stem cells (hMSC) were cultured in vitro on the scaffolds for up to 7 days. Our results demonstrate a stronger commitment toward early osteogenic differentiation of hMSC. Finally, we demonstrated that the increased in the specific surface area of the scaffold did not necessarily correlate with improved adsorption of protein and that other factors, such as surface chemistry and hydrophilicity, may also play a major role.
一种有前景的修复骨缺损的策略依赖于3D打印的多孔结构。尽管已经开展了多项研究来制造可控的、相互连通的多孔结构，但对于3D聚合物支架而言，在微观尺度及以下对表面特征进行控制仍然难以实现。在本研究中，我们开发并完善了一种方法，该方法可用于均匀且可重复地修饰聚合物3D打印支架的表面。我们已经证明，聚合物溶剂与超声的结合对于在不破坏结构完整性的情况下实现适当的表面修饰至关重要。支架上产生的修饰对支架的宏观和微观性质产生了深远影响，粗糙度增加、表面积增大且疏水性降低。此外，为了评估此类材料在骨组织工程中的性能，将人间充质干细胞（hMSC）在支架上体外培养长达7天。我们的结果表明hMSC在早期成骨分化方面表现出更强的倾向。最后，我们证明支架比表面积的增加不一定与蛋白质吸附的改善相关，其他因素，如表面化学性质和亲水性，也可能起主要作用。
