Liu Chang, Luo Yuxin, Wen Huijie, Qi Yanxia, Shi Guoyue, Deng Jingjing, Zhou Tianshu
School of Ecological and Environmental Sciences, Shanghai Key Lab for Urban Ecological Process and Eco-Restoration, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China and Institute of Eco-Chongming, 3663 North Zhongshan Road, Shanghai 20062, China.
School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
Nanoscale. 2021 Jan 21;13(2):1283-1290. doi: 10.1039/d0nr07449g.
Herein, combined with a pervasive smartphone installed with a color recognition app, dual-responsive CDs@Eu/GMP ICPs were designed as a red-to-blue paper-based colorimetric sensor for the point-of-use analysis of cerebral acetylcholinesterase (AChE) upon Cd2+ exposure. Blue-emitting CDs with multi-functional groups as guests were encapsulated into the network of Eu/GMP ICPs to obtain CDs@Eu/GMP ICPs with the sensitized red fluorescence of Eu3+. With the presence of thiocholine (TCh), derived from acetylthiocholine (ATCh) hydrolyzed by AChE, the coordination environment of the CDs@Eu/GMP ICPs was interrupted, leading to the collapse of the CDs@Eu/GMP ICP network and the corresponding release of guest CDs into the surrounding environment. Consequently, the sensitized red fluorescence of Eu3+ decreased and the blue fluorescence of the CDs increased. This obvious red-to-blue fluorescent color changes of CDs@Eu/GMP ICPs on test paper could then be integrated with the smartphone for point-of-use analysis of cerebral AChE upon Cd2+ exposure, which not only offers a new analytical platform for a better understanding of the environmental risk of Alzheimer's Dementia (AD), but also holds great potential in the early diagnosis of AD even at the asymptomatic stage with the decrease in CSF AChE as an early biomarker.
在此，结合安装了颜色识别应用程序的普及型智能手机，设计了双响应型CdS@Eu/GMP离子交联聚合物作为一种基于纸张的红到蓝比色传感器，用于在Cd2+暴露时对脑乙酰胆碱酯酶（AChE）进行即时分析。将具有多功能基团的蓝色发光硫化镉作为客体封装到Eu/GMP离子交联聚合物网络中，以获得具有Eu3+敏化红色荧光的CdS@Eu/GMP离子交联聚合物。在由AChE水解乙酰硫代胆碱（ATCh）衍生的硫代胆碱（TCh）存在下，CdS@Eu/GMP离子交联聚合物的配位环境被中断，导致CdS@Eu/GMP离子交联聚合物网络崩溃，客体硫化镉相应释放到周围环境中。因此，Eu3+的敏化红色荧光减弱，硫化镉的蓝色荧光增强。然后，试纸条上CdS@Eu/GMP离子交联聚合物这种明显的红到蓝荧光颜色变化可与智能手机结合，用于在Cd2+暴露时对脑AChE进行即时分析，这不仅为更好地了解阿尔茨海默病（AD）的环境风险提供了一个新的分析平台，而且在AD的早期诊断中甚至在无症状阶段也具有巨大潜力，以脑脊液AChE降低作为早期生物标志物。
