State Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, PR China.
School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, 230009, China.
Biosens Bioelectron. 2020 Jan 15;148:111791. doi: 10.1016/j.bios.2019.111791. Epub 2019 Oct 18.
A smartphone-integrated ratiometric fluorescent sensing system (DPA-Ce-GMP-Eu) for visual and point-of-care testing (POCT) of tetracycline with high sensitivity and accuracy was developed. The blue fluorescence of DPA-Ce-GMP was changed into red by doping with Eu duo to the energy transfer from Ce to Eu. Upon exposure to tetracycline, coordination between Eu and tetracycline blocks energy transfer from Ce to Eu, converting the fluorescent color from red to blue. The tetracycline detection can be realized within a wide concentration range from 0.01 μM to 45 μM. The limit of detection (LOD) reaches as low as 6.6 nM. To realize quantitative point-of-care detection in real samples, a portable device with smartphone as signal reader and analyzer is further designed to integrate with the DPA-Ce-GMP-Eu sensing platform. The Color Picker APP installed in the smartphone can convert the Red, Green and Blue (RGB) channels of the fluorescence images into digital values. With milk as real sample, tetracycline can be on-site detected with LOD of 10.8 nM. This developed platform presents a great promise for POCT in practical application with merits of low cost, easy carry, simple operation, and excellent selectivity and repeatability.
开发了一种智能手机集成的比率荧光传感系统（DPA-Ce-GMP-Eu），用于四环素的可视化和即时检测（POCT），具有高灵敏度和准确性。由于铈到铕的能量转移，DPA-Ce-GMP 的蓝色荧光通过掺杂 Eu 变成红色。暴露于四环素后，Eu 与四环素之间的配位阻止了铈到铕的能量转移，使荧光颜色从红色变为蓝色。四环素的检测可以在 0.01μM 至 45μM 的宽浓度范围内实现。检测限（LOD）低至 6.6nM。为了在实际样品中实现定量即时检测，进一步设计了一种带有智能手机作为信号读取器和分析仪的便携式设备，与 DPA-Ce-GMP-Eu 传感平台集成。智能手机中安装的 Color Picker APP 可以将荧光图像的红色、绿色和蓝色（RGB）通道转换为数字值。以牛奶为实际样品，四环素可以在现场以 10.8nM 的 LOD 进行检测。该开发平台具有成本低、携带方便、操作简单、选择性和重复性好等优点，有望在实际应用中实现即时检测。
