Chen H C, Peng S J, Gao H M, Su W T, Mei Y, Yi G L
Key Laboratory of Occupational Hazards Identification and Control in Hubei Province, School of Public Health, Wuhan University of Science and Technology, Wuhan 430065, China.
Wuhan Prevention and Treatment Center for Occupational Disease, Wuhan 430015, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2020 Dec 20;38(12):932-935. doi: 10.3760/cma.j.cn121094-20200102-00003.
To establish a headspace solid phase microextraction-gas chromatography method for determination of n-Butyl alcohol in urine. In October 2019, the n-butyl alcohol in urine was extracted with a polydimethylsiloxane/divinylbenzene (PDMS/DVB) solid-phase microextraction head. The conditions of salt amount, extraction temperature, extraction time and desorption time were optimized. The separation was performed on HP-5 (30 m×0.32 mm×0.25 μm) capillary column and detected with flame ionization detector. The quantification was based on the external standard curve. The linear relationship of n-butyl alcohol in urine was good in the range of 0.04-3.00 mg/L, the correlation coefficient was 0.999, the detection limit of the method was 0.04 mg/L, the recovery was 77.4%-102.8%, the intra-run precision was 3.67%-8.11%, and the inter-assay precision was 4.94%-6.90%. The method has simple operation, high concentration efficiency and high sensitivity, and it is suitable for the determination of n-butyl alcohol in urine of occupational exposure to n-butyl alcohol.
