Park Seon Young, Lee Mingyung, Lim Se Ra, Kwon Hyemin, Lee Ye Seul, Kim Ji Hyung, Seo Seongwon
Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.
Division of Animal and Dairy Sciences, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Korea.
Microorganisms. 2021 Jan 4;9(1):98. doi: 10.3390/microorganisms9010098.
/ complex (SBSEC) includes lactic acid-producing bacteria considered as the causative agent associated with acute rumen lactic acidosis in intensive ruminants. Considering the limited information on the detailed characteristics and diversity of SBSEC in Korea and the emergence of antimicrobial resistance (AMR), we investigated the diversity of SBSEC from domestic ruminants and verified the presence of antimicrobial resistance genes (ARGs) against several antimicrobials with their phenotypic resistance. Among 51 SBSEC isolates collected, two SBSEC members ( and ) were identified; -based phylogenetic analyses and comparisons of overall genome relatedness revealed potential plasticity and diversity. The AMR rates of these SBSEC against erythromycin, clindamycin, and tetracycline were relatively lower than those of other SBSEC isolates of a clinical origin. An investigation of the ARGs against those antimicrobials indicated that tetracycline resistance of SBSECs generally correlated with the presence of -possessing transposon. However, no correlation between the presence of ARGs and phenotypic resistance to erythromycin and clindamycin was observed. Although a limited number of animals and their SBSEC isolates were examined, this study provides insights into the potential intraspecies biodiversity of ruminant-origin SBSEC and the current status on antimicrobial resistance of the bacteria in the Korean livestock industry.
复杂（SBSEC）包括被认为是集约化反刍动物急性瘤胃酸中毒致病因素的产乳酸菌。考虑到韩国关于SBSEC详细特征和多样性的信息有限以及抗菌药物耐药性（AMR）的出现，我们调查了来自国内反刍动物的SBSEC的多样性，并通过其表型耐药性验证了针对几种抗菌药物的抗菌耐药基因（ARGs）的存在。在所收集的51株SBSEC分离株中，鉴定出了两个SBSEC成员（和）；基于的系统发育分析和全基因组相关性比较揭示了潜在的可塑性和多样性。这些SBSEC对红霉素、克林霉素和四环素的AMR率相对低于其他临床来源的SBSEC分离株。对这些抗菌药物的ARGs的调查表明，SBSECs对四环素的耐药性通常与携带转座子的存在相关。然而，未观察到ARGs的存在与对红霉素和克林霉素的表型耐药性之间的相关性。尽管检查的动物及其SBSEC分离株数量有限，但本研究为反刍动物来源的SBSEC潜在的种内生物多样性以及韩国畜牧业中该细菌的抗菌耐药性现状提供了见解。
