泪液自噬相关基因5作为干燥综合征诊断中一种潜在的新型生物标志物。

Tear ATG5 as a Potential Novel Biomarker in the Diagnosis of Sjögren Syndrome.

作者信息

Byun Yong-Soo, Lee Hyun Jung, Shin Soojung, Choi Moon Young, Kim Hyung-Seung, Chung So-Hyang

机构信息

Department of Ophthalmology and Visual Science, Catholic Institute for Visual Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.

Department of Biochemical Engineering, Seoul University, Seoul 02192, Korea.

出版信息

Diagnostics (Basel). 2021 Jan 4;11(1):71. doi: 10.3390/diagnostics11010071.

DOI:10.3390/diagnostics11010071

PMID:33406739

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7824715/

Abstract

Autophagy has been suggested to have an important role in the pathogenesis of Sjögren syndrome (SS). We previously identified that autophagy related 5 (ATG5) was elevated in the tear and conjunctival epithelial cells of SS dry eyes (DE) compared to non-SS DE. The purpose of this study was to investigate the role of tear ATG5 as a potential biomarker in the diagnosis of SS. To confirm this hypothesis, we evaluated the tear ATG5 concentration, and other ocular tests (Schirmer I, tear breakup time (TBUT), ocular surface staining (OSS) score, ocular surface disease index (OSDI)) in SS and non-DE, and compared their diagnostic performance to discriminate SS from non-SS DE. Tear ATG5 showed the greatest area under the curve (AUC = 0.984; 95% CI, 0.930 to 0.999) among the tests, and a 94.6% sensitivity and 93.6% specificity at a cutoff value of >4.0 ng/mL/μg. Our data demonstrated that tear ATG5 may be helpful as an ocular biomarker to diagnose and assess SS. In the future, the diagnostic power of tear ATG for SS should be validated.

摘要

自噬被认为在干燥综合征（SS）的发病机制中起重要作用。我们之前发现，与非干燥综合征干眼（DE）相比，干燥综合征干眼患者泪液和结膜上皮细胞中的自噬相关5（ATG5）水平升高。本研究的目的是探讨泪液ATG5作为潜在生物标志物在干燥综合征诊断中的作用。为证实这一假设，我们评估了干燥综合征患者和非干眼患者的泪液ATG5浓度以及其他眼部检查指标（泪液分泌试验I、泪膜破裂时间（TBUT）、眼表染色（OSS）评分、眼表疾病指数（OSDI）），并比较了它们区分干燥综合征与非干燥综合征干眼的诊断性能。在各项检查中，泪液ATG5的曲线下面积最大（AUC = 0.984；95%可信区间，0.930至0.999），在临界值>4.0 ng/mL/μg时，敏感性为94.6%，特异性为93.6%。我们的数据表明，泪液ATG5可能有助于作为诊断和评估干燥综合征的眼部生物标志物。未来，泪液ATG对干燥综合征的诊断能力应得到验证。