Matboli Marwa, Ibrahim Doaa, Hasanin Amany H, Hassan Mohamed K, Habib Eman K, Bekhet Miram M, Afifi Ahmed M, Eissa Sanaa
Department of Medical Biochemistry & Molecular Biology, Faculty of Medicine, Ain Shams University, Abbassia, PO Box 11381, Cairo, Egypt.
Department of Pharmacology, Faculty of Medicine, Ain Shams University, Abbassia, PO Box 11381, Cairo, Egypt.
Epigenomics. 2021 Feb;13(3):187-202. doi: 10.2217/epi-2020-0353. Epub 2021 Jan 7.
To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels. Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50 mg/kg/d) for 4 or 8 weeks. Fasting blood glucose, renal and lipid profiles were evaluated. Renal tissues were examined by light and electron microscopy. Autophagy genes ( and) and miR-15b, miR-34a and miR-633 were assessed by qRT-PCR, and LC3A/B by immunoblotting. Isorhamnetin improved fasting blood glucose, renal and lipid profiles with increased autophagosomes in renal tissues. It suppressed miRNA regulation of autophagy genes. We propose a molecular mechanism for the isorhamnetin renoprotective effect by modulation of autophagy epigenetic regulators.
为了通过研究异鼠李素在表观遗传、mRNA和蛋白质水平的作用，评估其对2型糖尿病大鼠模型糖尿病肾病的疗效。通过链脲佐菌素和高脂饮食诱导大鼠患2型糖尿病。用异鼠李素（50mg/kg/d）治疗大鼠4周或8周。评估空腹血糖、肾脏和血脂指标。通过光学和电子显微镜检查肾脏组织。通过qRT-PCR评估自噬基因（和）以及miR-15b、miR-34a和miR-633，通过免疫印迹评估LC3A/B。异鼠李素改善了空腹血糖、肾脏和血脂指标，同时肾组织中自噬体增加。它抑制了miRNA对自噬基因的调控。我们提出了异鼠李素通过调节自噬表观遗传调节因子发挥肾脏保护作用的分子机制。
