宫颈癌中高迁移率族蛋白B2（HMGB2）差异表达蛋白的鉴定及其临床病理意义

Identification of differentially expressed proteins and clinicopathological significance of HMGB2 in cervical cancer.

作者信息

Han Xiao, Zhong Siyi, Zhang Pengnan, Liu Yanmei, Shi Sangsang, Wu Congquan, Gao Shujun

机构信息

Center of Diagnosis and Treatment for Cervical Diseases, Obstetrics and Gynecology Hospital of Fudan University, No. 419, Fangxie Road, Huangpu District, Shanghai, 200011, China.

Shanghai Key Laboratory of Female Reproductive Endocrine-Related Disease, Fudan University, Shanghai, 200011, China.

出版信息

Clin Proteomics. 2021 Jan 6;18(1):2. doi: 10.1186/s12014-020-09308-4.

DOI:10.1186/s12014-020-09308-4

PMID:33407071

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7789524/

Abstract

To investigate the complexity of proteomics in cervical cancer tissues, we used isobaric tags for relative and absolute quantitation (iTRAQ)-based mass spectrometry analysis on a panel of normal cervical tissues (N), high-grade squamous intraepithelial lesion tissues (HSIL) and cervical cancer tissues (CC). Total 72 differentially expressed proteins were identified both in CC vs N and CC vs HSIL. The expression of HMGB2 was markedly higher in CC than that in HSIL and N. High HMGB2 expression was significantly correlated with primary tumor size, invasion and tumor stage. The up-regulated HMGB2 was discovered to be associated with human cervical cancer. These findings suggest that HMGB2 may be a potentially prognostic biomarker and a target for the therapy of cervical cancer.

摘要

为了研究宫颈癌组织中蛋白质组学的复杂性，我们使用基于等压标签相对和绝对定量（iTRAQ）的质谱分析方法，对一组正常宫颈组织（N）、高级别鳞状上皮内病变组织（HSIL）和宫颈癌组织（CC）进行了分析。在CC与N以及CC与HSIL的比较中，共鉴定出72种差异表达蛋白。HMGB2在CC中的表达明显高于HSIL和N。HMGB2高表达与原发肿瘤大小、侵袭及肿瘤分期显著相关。研究发现上调的HMGB2与人类宫颈癌有关。这些结果表明，HMGB2可能是一种潜在的预后生物标志物和宫颈癌治疗的靶点。