Hill C K, Holland J, Chang-Liu C M, Buess E M, Peak J G, Peak M J
Division of Biological and Medical Research, Argonne National Laboratory, Illinois 60439.
Radiat Res. 1988 Feb;113(2):278-88.
Survival parameters and immediate DNA damage induced by 60Co gamma rays, 50-kVp X rays, and Janus fission-spectrum neutrons in human epithelial P3 cells (derived from an embryonic teratocarcinoma) are compared with those for Chinese hamster lung V79 cells. DNA damage caused by X and gamma irradiation, measured by alkaline elution methods, is the same in both cell types, whereas the P3 cells are about two times more sensitive (as measured by Do ratios of the final survival curve slope) to the lethal effects of these radiations than are the V79 cells. Human P3 cells are also more sensitive to the lethal effects of fission-spectrum neutrons than V79 cells. Survival experiments with split radiation doses and hypertonic salt treatment indicate that both P3 cells and V79 cells can recover from radiation-induced damage efficiently.
将人类上皮P3细胞（源自胚胎性畸胎癌）中由60Coγ射线、50 kVp X射线和贾纳斯裂变谱中子诱导的存活参数及即时DNA损伤，与中国仓鼠肺V79细胞的相应参数进行比较。通过碱性洗脱法测量，X射线和γ射线照射引起的DNA损伤在两种细胞类型中是相同的，而P3细胞对这些辐射的致死效应的敏感性（以最终存活曲线斜率的Do比值衡量）约为V79细胞的两倍。人类P3细胞对裂变谱中子的致死效应也比V79细胞更敏感。分割辐射剂量和高渗盐处理的存活实验表明，P3细胞和V79细胞都能有效地从辐射诱导的损伤中恢复。
