School of Pharmacy and Life Sciences, Robert Gordon University, Sir Ian Wood Building, Garthdee Road, Aberdeen, AB10 7GJ, Scotland.
Graduate School, Robert Gordon University, The Ishbel Gordon Building, Garthdee Road, Aberdeen, AB10 7QE, Scotland.
BMC Res Notes. 2021 Jan 6;14(1):8. doi: 10.1186/s13104-020-05418-2.
The urgent need for novel antibiotics cannot be overemphasized. Hafnia alvei A23BA was isolated from plant rhizosphere as part of an effort to recover novel antibiotic-producing bacterial strains from soil samples. The genome of the isolate was sequenced to facilitate mining for potential antibiotic-encoding biosynthetic gene clusters and to gain insights into how these gene clusters could be activated.
Here, we report the complete genome sequence of H. alvei A23BA obtained from the hybrid assembly of Illumina HiSeq and GridION reads. The genome, consisting of a circular chromosome and a circular plasmid, is 4.77 Mb in size with a GC content of 48.77%. The assembly is 99.5% complete with genomic features including 4,217 CDSs, 125 RNAs, and 30 pseudogenes. Thiopeptide, beta-lactone, siderophore, and homoserine lactone biosynthetic gene clusters were also identified. Other gene clusters of interest include those associated with bioremediation, biocontrol, and plant growth promotion- all of which are reported for H. alvei for the first time. This dataset serves to expedite the exploration of the biosynthetic and metabolic potentials of the species. Furthermore, being the first published genome sequence of a soil isolate, this dataset enriches the comparative genomics study of H. alvei strains.
新型抗生素的迫切需求怎么强调都不为过。海氏不动杆菌 A23BA 是从植物根际中分离出来的，这是从土壤样本中恢复新型抗生素产生菌株的努力的一部分。对该分离株的基因组进行测序，以方便挖掘潜在的抗生素编码生物合成基因簇，并深入了解如何激活这些基因簇。
在这里，我们报告了从 Illumina HiSeq 和 GridION 读取的混合组装中获得的海氏不动杆菌 A23BA 的完整基因组序列。该基因组由一个圆形染色体和一个圆形质粒组成，大小为 4.77 Mb，GC 含量为 48.77%。组装的完整性为 99.5%，具有 4217 个 CDS、125 个 RNA 和 30 个假基因等基因组特征。还鉴定了硫肽、β-内酰胺、铁载体和同型乳酰基因簇。其他感兴趣的基因簇包括与生物修复、生物防治和植物生长促进相关的基因簇——所有这些都是海氏不动杆菌首次报道的。该数据集有助于加速该物种生物合成和代谢潜力的探索。此外，作为土壤分离株的第一个公布的基因组序列，该数据集丰富了海氏不动杆菌菌株的比较基因组学研究。
