Department of Materials, Imperial College London, London SW7 2AZ, UK.
Department of Bioengineering, Imperial College London, London SW7 2AZ, UK.
Theranostics. 2021 Jan 1;11(4):2006-2019. doi: 10.7150/thno.53031. eCollection 2021.
Theranostics, the combination of diagnosis and therapy, has long held promise as a means to achieving personalised precision cancer treatments. However, despite its potential, theranostics has yet to realise significant clinical translation, largely due the complexity and overriding toxicity concerns of existing theranostic nanoparticle strategies. Here, we present an alternative nanoparticle-free theranostic approach based on simultaneous Raman spectroscopy and photodynamic therapy (PDT) in an integrated clinical platform for cancer theranostics. We detail the compatibility of Raman spectroscopy and PDT for cancer theranostics, whereby Raman spectroscopic diagnosis can be performed on PDT photosensitiser-positive cells and tissues without inadvertent photosensitiser activation/photobleaching or impaired diagnostic capacity. We further demonstrate that our theranostic platform enables tumour diagnosis, treatment, and post-treatment molecular monitoring in real-time. This system thus achieves effective theranostic performance, providing a promising new avenue towards the clinical realisation of theranostics.
治疗诊断学，即将诊断与治疗相结合，一直以来都被寄予厚望，有望实现针对癌症的个体化精准治疗。然而，尽管它有潜力，但治疗诊断学尚未实现显著的临床转化，主要是因为现有的治疗诊断学纳米颗粒策略的复杂性和毒性问题。在这里，我们提出了一种基于同时拉曼光谱和光动力疗法（PDT）的纳米颗粒自由治疗诊断学的替代方法，该方法整合到一个临床平台中用于癌症的治疗诊断学。我们详细介绍了拉曼光谱和 PDT 在癌症治疗诊断学中的兼容性，即拉曼光谱诊断可以在 PDT 光敏剂阳性的细胞和组织上进行，而不会意外地激活/光漂白光敏剂或损害诊断能力。我们还进一步证明，我们的治疗诊断学平台可以实时实现肿瘤的诊断、治疗和治疗后的分子监测。因此，该系统实现了有效的治疗诊断学性能，为治疗诊断学的临床实现提供了一条有前途的新途径。
