Bertechini Antonio G, Dalolio Felipe Santos, Carvalho Julio C C, Carvalho Andressa C, Sorbara Jose O B
Animal Science Department, Federal University of Lavras, Minas Gerais, Brazil.
DSM Nutritional Products, São Paulo, Brazil.
Transl Anim Sci. 2020 Nov 27;4(4):txaa219. doi: 10.1093/tas/txaa219. eCollection 2020 Oct.
This experiment was carried out to study the effect of dietary exogenous monocomponent protease on the coefficient apparent total tract (ATTD) and apparent ileal (AID) digestibility of amino acids of corn, soybean meal (SBM), and full fat soybean meal (FFSM) in broilers. A total of 400 males Cobb-500 (14 d of age) were equally allocated in 80 metabolic cages (50 cm × 50 cm × 45 cm) in a completely randomized design and a semi-controlled environment. Eight treatments (basal diet with or without a protease and three ingredients replacing the basal diets in 40% to corn and 30% to SBM and FFSM, with and without protease), with 10 replicates each were evaluated. The protease was added at 200 mg/kg resulting in 15,000 unit of PROT/kg. The total collection of excreta was held during 3 d, after 5 d for adaptation of broilers at the diets. The ileal content was collected on d 21, after slaughter of birds. The enzyme increased ( < 0.05) the ATTD and AID of most amino acids contained in SBM and FFSM. The digestibility of cysteine, glycine, proline, and threonine had higher ( < 0.05) ATTD and AID in all tested ingredients with the use of protease. On average, the dietary protease increased in 5.19% and 3.86% the total and ileal digestibility of amino acids, respectively. It was concluded that the dietary protease exerts major effects on toasted full-fat soybean, followed by soybean meal and corn to broilers.
本试验旨在研究日粮中外源单一组分蛋白酶对肉鸡玉米、豆粕（SBM）和全脂豆粕（FFSM）氨基酸表观全肠道（ATTD）和表观回肠（AID）消化率的影响。总共400只14日龄的雄性科宝500肉鸡被完全随机分配到80个代谢笼（50 cm×50 cm×45 cm）中，饲养于半控制环境。试验设8个处理（基础日粮添加或不添加蛋白酶，以及用三种原料分别替代40%玉米和30%豆粕与全脂豆粕的基础日粮，添加或不添加蛋白酶），每个处理10个重复。蛋白酶添加量为200 mg/kg，相当于15,000单位PROT/kg。在肉鸡适应日粮5 d后，进行3 d的排泄物全收粪。在第21天屠宰肉鸡后收集回肠内容物。该酶提高了（P<0.05）豆粕和全脂豆粕中大多数氨基酸的ATTD和AID。在所有测试原料中，使用蛋白酶后，半胱氨酸、甘氨酸、脯氨酸和苏氨酸的消化率具有更高（P<0.05）的ATTD和AID。日粮蛋白酶使氨基酸的总消化率和回肠消化率分别平均提高了5.19%和 3.86%。由此得出结论，日粮蛋白酶对肉鸡烤全脂大豆的影响最大，其次是豆粕和玉米。
