Bertechini Antonio G, de Carvalho Júlio C C, Carvalho Andressa C, Dalolio Felipe S, Sorbara Jose O B
Animal Science Department, Federal University of Lavras, Lavras, Minas Gerais, Brazil.
Technical Support-DSM Nutritional Products, São Paulo, São Paulo, Brazil.
Transl Anim Sci. 2020 Oct 9;4(4):txaa187. doi: 10.1093/tas/txaa187. eCollection 2020 Oct.
A digestibility experiment was conducted to evaluate the effects of dietary exogenous monocomponent protease on the coefficient of apparent total tract digestibility (ATTD) and coefficient apparent ileal digestibility (AID) of amino acids in meat and bones meal (MBM), poultry byproducts meal (PBPM), and feather meal (FM). A total of 512 Cobb-500 male broiler chickens (aged 14 d) were randomly placed into 64 metabolism cages (8 birds per pen) and were allocated to eight treatments with eight replicates in a semi-controlled environmental room. The experimental diets consisted of the basal diet (corn/SBM) and the replacement of 300 g/kg on a weight basis with MBM, PBPM, or FM. The excreta were collected during 3 d (19 to 21 d), and the ileal digesta (using Celite as an indigestible marker) only 1 d (21 d). The protease contained 75,000 PROT units/g. The use of the enzyme increased ( 0.05) ATTD for alanine, cysteine, glycine, and threonine in the basal diet and AID for the amino acids alanine, cysteine, glycine, lysine, threonine, and valine for the basal diet (vegetable). Regarding meals, there was an increase ( 0.05) in the amino acid digestibility in large part due to the amino acids of MBM (14) and PBPM (9), with only five amino acids for FM. The amino acids glycine and threonine showed increases ( 0.05) in both total and ileal digestibility for all animal meals and for the diet based on corn and soybean meal, which indicates a high specificity of the enzyme for these amino acids. The overall results obtained in this study found satisfactory effectiveness of this exogenous protease. The total collection method was lower by 1.83% of amino acids digestibility than the ileal method.
进行了一项消化率试验，以评估日粮中外源单组分蛋白酶对肉骨粉（MBM）、禽副产品粉（PBPM）和羽毛粉（FM）中氨基酸的表观全肠道消化率（ATTD）和表观回肠消化率（AID）的影响。总共512只14日龄的科宝500雄性肉鸡被随机放入64个代谢笼（每栏8只鸡），并在半控制环境室内分配到8个处理组，每组8个重复。试验日粮由基础日粮（玉米/豆粕）组成，并以重量计用MBM、PBPM或FM替代300 g/kg。在3天（19至21天）内收集排泄物，仅在1天（21天）收集回肠食糜（使用硅藻土作为不可消化标记物）。该蛋白酶含有75,000 PROT单位/克。酶的使用使基础日粮中丙氨酸、半胱氨酸、甘氨酸和苏氨酸的ATTD增加（P<0.05），以及基础日粮（植物性）中丙氨酸、半胱氨酸、甘氨酸、赖氨酸、苏氨酸和缬氨酸的AID增加（P<0.05）。对于各粕类，氨基酸消化率有所提高（P<0.05），这在很大程度上归因于MBM（14种）和PBPM（9种）中的氨基酸，而FM中只有5种氨基酸。甘氨酸和苏氨酸在所有动物粕类以及基于玉米和豆粕的日粮中的总消化率和回肠消化率均有所提高（P<0.05），这表明该酶对这些氨基酸具有高度特异性。本研究获得的总体结果表明这种外源蛋白酶具有令人满意的效果。总收集法测得的氨基酸消化率比回肠法低1.83%。
