Bai Manyuan, Wang Rui, Sun Shiqi, Zhang Yun, Dong Hu, Guo Huichen
State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, 730046, Gansu, People's Republic of China.
College of Animal Science, Yangtze University, Jingmi Street, Jingzhou District, Jingzhou, 434025, People's Republic of China.
AMB Express. 2021 Jan 6;11(1):7. doi: 10.1186/s13568-020-01167-4.
Virus-like particles (VLPs) are high-priority antigens with highly ordered repetitive structures, which are similar to natural viral particles. We have developed a competitive enzyme-linked immunosorbent assay (cELISA) for detecting antibodies directed against Senecavirus A (SVA). Our assay utilizes SVA VLPs that were expressed and assembled in an E. coli expression system as the coating antigens. VLPs have better safety and immunogenicity than intact viral particles or peptides. The VLPs-based cELISA was used to test 342 serum samples collected from different pig farms, and the results showed that its specificity and sensitivity were 100% and 94%, respectively. The consistency rates of cELISA with the BIOSTONE AsurDx™ Senecavirus A (SVA) Antibody Test Kit and an indirect immunofluorescent assay were 90.0% and 94.2%, respectively. Therefore, this VLPs-based cELISA can be effectively and reliably used for the detection and discrimination of SVA infection in serum samples.
病毒样颗粒（VLPs）是具有高度有序重复结构的高优先级抗原，与天然病毒颗粒相似。我们开发了一种竞争性酶联免疫吸附测定（cELISA）来检测针对猪捷申病毒A（SVA）的抗体。我们的测定使用在大肠杆菌表达系统中表达和组装的SVA VLPs作为包被抗原。VLPs比完整病毒颗粒或肽具有更好的安全性和免疫原性。基于VLPs的cELISA用于检测从不同猪场收集的342份血清样本，结果表明其特异性和敏感性分别为100%和94%。cELISA与BIOSTONE AsurDx™猪捷申病毒A（SVA）抗体检测试剂盒和间接免疫荧光测定的一致性率分别为90.0%和94.2%。因此，这种基于VLPs的cELISA可有效、可靠地用于血清样本中SVA感染的检测和鉴别。
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