Silverman D G, Kim D J, Brousseau D A, Kim M, Norton K J, Reilly C A
Anesthesia and Surgical Research Laboratories, Philadelphia Veterans Administration Medical Center, Pa.
Surgery. 1988 Feb;103(2):221-5.
This study evaluated oral ingestion as the route of fluorescein administration for fluorescence assessment of skin perfusion. In 10 anesthetized rats, dorsal pedicle flaps were raised to produce graded perfusion; then fluorescein (7.5 mg/kg) was administered via an oropharyngeal tube. Quantification of skin fluorescence, performed with the fiberoptic fluorometer 45 minutes after dye ingestion, delineated a significant difference between the perfusion of flap regions that remained viable and those that subsequently became dystrophic (p less than 0.05). The precision was equivalent to that previously reported after intravenous dye. In addition, the gradual delivery of dye after oral administration permitted monitoring of slope of uptake and time to peak. Each of these pharmacokinetic parameters delineated a significant difference between viable and nonviable sections. As measures of relative change in fluorescence, they are independent of skin color and thickness. This feature suggests that the oral route actually may improve the accuracy and reliability of the fluorescein test.
本研究评估了口服荧光素作为给药途径用于皮肤灌注荧光评估的效果。在10只麻醉大鼠中，掀起背部带蒂皮瓣以产生不同程度的灌注；然后通过口咽管给予荧光素（7.5毫克/千克）。在摄入染料45分钟后，用光纤荧光计对皮肤荧光进行定量分析，结果显示存活皮瓣区域与随后发生营养不良的皮瓣区域的灌注存在显著差异（p小于0.05）。其精确度与先前静脉注射染料后的报告相当。此外，口服给药后染料的逐渐释放使得能够监测摄取斜率和达到峰值的时间。这些药代动力学参数中的每一个都显示了存活和非存活部分之间的显著差异。作为荧光相对变化的测量指标，它们不受皮肤颜色和厚度的影响。这一特性表明口服途径实际上可能会提高荧光素测试的准确性和可靠性。
