二氯甲烷在灌注大鼠肝脏中的代谢及其产物一氧化碳与细胞色素P-450的结合。

Metabolism of dichloromethane and the subsequent binding of its product, carbon monoxide, to cytochrome P-450 in perfused rat liver.

作者信息

Takano T, Miyazaki Y

机构信息

Department of Public Health and Environmental Science, Faculty of Medicine, Tokyo Medical and Dental University, Japan.

出版信息

Toxicol Lett. 1988 Jan;40(1):93-6. doi: 10.1016/0378-4274(88)90187-7.

DOI:10.1016/0378-4274(88)90187-7

PMID:3341052

Abstract

The interaction of cytochrome P-450 and dichloromethane (DCM) was investigated spectrophotometrically in the perfused rat liver. DCM bound to cytochrome P-450 and showed a type I spectral change, with a peak at 450 nm. The peak at 450 nm was developed by addition of exogenous carbon monoxide (CO) to the perfusate. By examining the pattern of the spectral change, we concluded that CO formed during DCM metabolism by cytochrome P-450 bound to cytochrome P-450 in the perfused liver.

摘要

在灌注大鼠肝脏中，采用分光光度法研究了细胞色素P - 450与二氯甲烷（DCM）的相互作用。DCM与细胞色素P - 450结合并呈现I型光谱变化，在450 nm处有一个峰值。通过向灌注液中添加外源性一氧化碳（CO），450 nm处的峰值得以显现。通过检查光谱变化模式，我们得出结论，在灌注肝脏中，细胞色素P - 450代谢DCM过程中形成的CO与细胞色素P - 450结合。