Institute of Glycobiochemistry, National Engineering Research Centre of Chemical Fertilizer Catalyst, Fu Zhou University, Fu Zhou, 350002, People's Republic of China.
College of Biological Science and Engineering, Fu Zhou University, Fu Zhou, 350002, People's Republic of China.
Glycoconj J. 2021 Feb;38(1):25-33. doi: 10.1007/s10719-020-09962-8. Epub 2021 Jan 7.
Oviductus ranae (O.ran.) has been widely used as a tonic and a traditional animal-based Chinese medicine. O.ran. extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic. Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities. However, their presence and possible structural characteristics within O.ran. were previously unknown. In this study, glycosaminoglycans were isolated from O.ran. and their disaccharide compositions were analyzed by liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF). Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran. with varied disaccharide compositions. HS species contain highly acetylated disaccharides, and have various structures in their constituent chains. CS/DS chains also possess a heterogeneous structure with different sulfation patterns and densities. This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of O.ran..
蛙输卵管（O.ran.）被广泛用作滋补品和传统动物源中药。据报道，O.ran.提取物具有多种生物活性，包括经常与哺乳动物糖胺聚糖相关的活性，如抗炎、抗骨质疏松和抗哮喘。糖胺聚糖是哺乳动物中普遍存在的复杂线性多糖，具有广泛的生物活性。然而，它们在 O.ran.中的存在和可能的结构特征以前是未知的。在这项研究中，从 O.ran.中分离出糖胺聚糖，并通过液相色谱-离子阱/飞行时间质谱联用仪（LC-MS-ITTOF）分析其二糖组成。在 O.ran.中检测到了肝素硫酸盐（HS）/肝素（HP）、硫酸软骨素（CS）/硫酸皮肤素（DS）和透明质酸（HA），它们具有不同的二糖组成。HS 物种含有高度乙酰化的二糖，其组成链具有不同的结构。CS/DS 链也具有异质结构，具有不同的硫酸化模式和密度。这些新的结构信息可能有助于阐明这些多糖在 O.ran.生物活性中的可能作用。
