人脑中动脉酸性糖胺聚糖的组成
Composition of acidic glycosaminoglycans in human cerebral arteries.
作者信息
Murata K, Nakazawa K
出版信息
Atherosclerosis. 1976 Oct;25(1):31-43. doi: 10.1016/0021-9150(76)90045-9.
(1) The composition of acidic glycosaminoglycans (AGAG) in the pooled human cerebral arteries was investigated by electrophoretic characterization before and after digestion with chondroitinases. Constitution of the chondroitin sulfate (CS) family was determined qualitatively on the basis of the disaccharide subunits of the CS chains after depolymerization with the enzymes. (2) The data obtained indicated that the AGAG in cerebral arteries consisted of, in order of amount, heparan sulfates, chondroitin-6-sulfate, dermatan sulfate, chondroitin-4-sulfate and hyaluronic acid. (3) The existence of oversulfated CS and undersulfated CS in the cerebral AGAG was supported by the detection of unsaturated di-sulfated and non-sulfated disaccharides on paper chromatography. In addition, the presence of hyaluronic acid was indicated by electrophoretic and enzymatic separation. (4) The distribution of the individual AGAG in cerebral arteries was also examined on the basis of molecular weight by gel filtration on a Sephadex G-100 column.
(1) 在用软骨素酶消化前后，通过电泳表征研究了汇集的人脑动脉中酸性糖胺聚糖（AGAG）的组成。在用这些酶解聚后，根据硫酸软骨素（CS）链的二糖亚基定性确定硫酸软骨素（CS）家族的组成。(2) 获得的数据表明，脑动脉中的AGAG按含量依次由硫酸乙酰肝素、硫酸软骨素-6-硫酸酯、硫酸皮肤素、硫酸软骨素-4-硫酸酯和透明质酸组成。(3) 通过纸色谱法检测到不饱和二硫酸化和非硫酸化二糖，支持了脑AGAG中存在过度硫酸化的CS和硫酸化不足的CS。此外，通过电泳和酶分离表明存在透明质酸。(4) 还通过在Sephadex G-100柱上进行凝胶过滤，根据分子量检查了脑动脉中各个AGAG的分布。
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