Department of Ophthalmology, Chungbuk National University Hospital, College of Medicine, Chungbuk National University, Cheongju, Korea; and.
Departments of Ophthalmology, and.
Retina. 2021 Aug 1;41(8):1715-1722. doi: 10.1097/IAE.0000000000003099.
To determine the relationship of drusen size as determined by spectral-domain optical coherence tomography (SD-OCT) with that measured on registered color fundus photography (CFP) images and to derive an OCT-based classification system that was comparable with that determined by CFP.
Custom software was developed to register CFP images to the scanning laser ophthalmoscopy fundus images obtained simultaneously with the corresponding SD-OCT images, so that individual drusen observed on CFP could be matched with those seen on SD-OCT. Single druse size (diameter, area, volume, and height) on CFP and SD-OCT images from a phase two clinical trial was determined with the Duke OCT Retinal Analysis Program.
The sizes of 213 individual drusen were measured on CFP and SD-OCT. The drusen diameter measured on CFP was significantly correlated with those determined on SD-OCT (R: 0.879, P < 0.001). Based on the corresponding formula: drusen diameter on SD-OCT = 0.77 × (drusen diameter on CFP) + 50.67 µm, large drusen defined as ≥125 µm on CFP had a diameter ≥145 µm on OCT, medium drusen defined as 63 µm to 124 µm on CFP had diameters 100 µm to 144 µm on OCT, and small drusen defined as <63 µm on CFP had diameters <100 µm on OCT.
With our registration software and imaging processing algorithms, we were able to correlate individual druse sizes measured on CFP with those determined on SD-OCT. These data can be used to develop an SD-OCT-based grading scale, analogous to the CFP Age-Related Eye Disease Study drusen scale that may be useful in the clinic and in clinical trials.
确定用谱域光相干断层扫描（SD-OCT）测量的玻璃膜疣大小与用已注册的彩色眼底照相（CFP）图像测量的大小之间的关系，并建立一种与 CFP 确定的方法可比的基于 OCT 的分类系统。
开发了定制软件，将 CFP 图像与同时获得的扫描激光检眼镜眼底图像以及相应的 SD-OCT 图像进行配准，以便在 CFP 上观察到的单个玻璃膜疣与在 SD-OCT 上观察到的玻璃膜疣相匹配。使用 Duke OCT 视网膜分析程序确定来自第二阶段临床试验的 CFP 和 SD-OCT 图像上的单个玻璃膜疣大小（直径、面积、体积和高度）。
在 CFP 和 SD-OCT 上测量了 213 个单独的玻璃膜疣的大小。CFP 上测量的玻璃膜疣直径与 SD-OCT 上测量的直径显著相关（R：0.879，P<0.001）。根据相应的公式：SD-OCT 上的玻璃膜疣直径=0.77×（CFP 上的玻璃膜疣直径）+50.67µm，CFP 上定义的大玻璃膜疣（≥125µm）在 OCT 上的直径≥145µm，CFP 上定义的中等玻璃膜疣（63µm 至 124µm）在 OCT 上的直径为 100µm 至 144µm，而 CFP 上定义的小玻璃膜疣（<63µm）在 OCT 上的直径<100µm。
使用我们的配准软件和成像处理算法，我们能够将 CFP 上测量的单个玻璃膜疣大小与 SD-OCT 上测量的大小相关联。这些数据可用于开发基于 SD-OCT 的分级量表，类似于 CFP 年龄相关性眼病研究玻璃膜疣量表，这可能在临床和临床试验中有用。
