Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Exp Parasitol. 2021 Mar;222:108063. doi: 10.1016/j.exppara.2020.108063. Epub 2021 Jan 4.
Leishmaniasis is one of the most neglected tropical infectious diseases in the world. The emergence of drug resistance and toxicity and the high cost of the available drugs with a lack of new anti-leishmanial drugs highlight the need to search for newer therapies with anti-leishmanial activities. Due to the mesenchymal stem cell (MSC) immunomodulatory capacity, they have been applied in a wide variety of disorders. In this study, the potential effects of adipose-derived MSC (AD-MSCs) therapy and its combination with glucantime were evaluated in a murine model of cutaneous leishmaniasis induced by L. major. The results showed that AD-MSCs improved wound healing and decreased parasite burden. The real-time PCR results obtained from mice treated with AD-MSCs showed that IL-12 and TNF-α genes were upregulated. IL-10, arginase, and FOXP3 genes were downregulated whereas no differences in expression of the IL-4 gene were found. Overall, it seems that AD-MSCs therapy enhances Th1 immune response in L. major infected BALB/c mice. Unexpectedly, our results showed that the association of glucantime to AD-MSCs treatments did not lead to an increment in the anti-leishmanial activity.
利什曼病是世界上最被忽视的热带传染病之一。耐药性和毒性的出现以及现有药物的高成本，加上缺乏新的抗利什曼病药物，突出表明需要寻找具有抗利什曼病活性的新疗法。由于间充质干细胞 (MSC) 的免疫调节能力，它们已被广泛应用于各种疾病。在这项研究中，评估了脂肪来源的 MSC (AD-MSCs) 治疗及其与葡萄糖胺合用在 L. major 诱导的皮肤利什曼病小鼠模型中的潜在作用。结果表明 AD-MSCs 改善了伤口愈合并减少了寄生虫负担。从用 AD-MSCs 治疗的小鼠中获得的实时 PCR 结果显示，IL-12 和 TNF-α 基因上调。IL-10、精氨酸酶和 FOXP3 基因下调，而 IL-4 基因的表达没有差异。总的来说，AD-MSCs 治疗似乎增强了 L. major 感染 BALB/c 小鼠的 Th1 免疫反应。出乎意料的是，我们的结果表明，葡萄糖胺与 AD-MSCs 治疗的联合并未导致抗利什曼病活性的增加。
